Chitinases or chitinolytic enzymes have different applications in the field of medicine, agriculture, and industry. The present study is aimed at developing an effective hyperchitinase-producing mutant strain of novel Bacillus licheniformis. A simple and rapid methodology was used for screening potential chitinolytic microbiota by chemical mutagenesis with ethylmethane sulfonate and irradiation with UV. There were 16 mutant strains exhibiting chitinase activity. Out of the chitinase-producing strains, the strain with maximum chitinase activity was selected, the protein was partially purified by SDS-PAGE, and the strain was identified as Bacillus licheniformis (SSCL-10) with the highest specific activity of 3.4 U/mL. The induced mutation model has been successfully implemented in the mutant EMS-13 (20.2 U/mL) that produces 5-6-fold higher yield of chitinase, whereas the mutant UV-11 (13.3 U/mL) has 3-4-fold greater chitinase activity compared to the wild strain. The partially purified chitinase has a molecular weight of 66 kDa. The wild strain (SSCL-10) was identified as Bacillus licheniformis using 16S rRNA sequence analysis. This study explores the potential applications of hyperchitinase-producing bacteria in recycling and processing chitin wastes from crustaceans and shrimp, thereby adding value to the crustacean industry.

中文翻译：

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用于降解几丁质的新分离的几丁质酶生产菌株地衣芽孢杆菌 SSCL-10 的鉴定和表征

几丁质酶或几丁质分解酶在医学、农业和工业领域有不同的应用。本研究旨在开发一种有效的产金丝桃素酶的新型地衣芽孢杆菌突变株。一种简单快速的方法用于通过甲磺酸乙酯化学诱变和紫外线照射筛选潜在的几丁质分解微生物群。有16个突变株表现出几丁质酶活性。从产生几丁质酶的菌株中选出几丁质酶活性最大的菌株，用SDS-PAGE部分纯化蛋白，鉴定为地衣芽孢杆菌(SSCL-10) 的最高比活为 3.4 U/mL。诱导突变模型已在突变体 EMS-13 (20.2 U/mL) 中成功实施，其产生的几丁质酶产量提高了 5-6 倍，而突变体 UV-11 (13.3 U/mL) 的产量提高了 3-4 倍与野生菌株相比具有更高的几丁质酶活性。部分纯化的几丁质酶的分子量为 66 kDa。使用 16S rRNA 序列分析将野生菌株 (SSCL-10) 鉴定为地衣芽孢杆菌。本研究探讨了产生超几丁质酶的细菌在回收和加工甲壳类和虾类甲壳类废物中的潜在应用，从而为甲壳类行业增加价值。