Abstract

In the current research, a new Cu(II)-containing coordination polymer with the chemical formula of [Cu(IPT)₂]_n (1) has been successfully prepared by reaction of the Cu(NO₃)₂·3H₂O with a heterotopic imidazole-tetrazole-bifunctional ligand 5-[3-(1H-imidazol-1-yl)phenyl]-1H-tetrazolate. Furthermore, its treatment activity on the diabetic retinopathy was assessed and the related mechanism was discussed at the same time. First of all, the real time RT-PCR was conducted and the expression levels of the bcl-2 gene in retinal cells were measured after compound treatment. Besides, the cell apoptosis assay was also performed, and the percentage of the apoptotic retinal cells were determined after compound exposure. Molecular docking simulation shows that its bioactivity is from five-membered ring containing nitrogen atoms.

摘要

在目前的研究中，通过Cu(NO ₃ ) ₂ ·3H ₂ O与化学式[Cu(IPT) ₂ ] _n ( 1 )异位咪唑-四唑-双功能配体5-[3-(1H-咪唑-1-基)苯基]-1H-四唑。此外，还评估了其对糖尿病视网膜病变的治疗活性，并同时讨论了相关机制。首先，进行实时 RT-PCR 和bcl-2的表达水平在化合物处理后测量视网膜细胞中的基因。此外，还进行了细胞凋亡实验，测定了化合物暴露后视网膜细胞凋亡的百分比。分子对接模拟表明其生物活性来自含氮原子的五元环。