Abstract

Dexamethasone is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of melatonin on testis histological changes and Spermatogenesis indexes in adult mice following treatment with dexamethasone. Adult male NMRI mice were divided randomly into four groups: control, dexamethasone (i.p injections, 7 mg/kg/day), dexamethasone + melatonin and melatonin (i.p injections, 20 mg/kg/day). After 7 days of treatment, the right testes were studied stereologically and the left testes were used to measure the daily sperm production (DSP). The serum levels of malondialdehyde (MDA), testosterone and total antioxidant capacity (TAC) were also measured. The left caudal epididymis was used to analyze sperm parameters. Data were analyzed using one way ANOVA and means were considered significantly different at p < 0.05. A significant decrease in the testis volume, seminiferous tubules volume, the number of spermatocytes, round and long spermatids, Spermatogenesis indexes, sperm parameters such as motility, count, viability, tail length and DSP, serum testosterone level, TAC and the body weight was found in the dexamethasone group compared to the control. Meanwhile a significant swelling of the interstitial tissue and a significant increase in the MDA level was found in the dexamethasone group compared to the control. The above parameters reached the control level in the dexamethasone + melatonin group. Melatonin can compensate for the adverse effects of dexamethasone on sperm parameters and the histology of the seminiferous tubules in mice.

摘要

地塞米松是一种常见的药物，能够导致男性生殖系统畸形。本研究的目的是研究褪黑激素对地塞米松治疗后成年小鼠睾丸组织学变化和精子发生指标的影响。成年雄性 NMRI 小鼠随机分为四组：对照组、地塞米松（腹腔注射，7 mg/kg/天）、地塞米松+褪黑激素和褪黑激素（腹腔注射，20 mg/kg/天）。治疗7天后，对右侧睾丸进行体视学研究，左侧睾丸用于测量每日精子产量（DSP）。还测量了丙二醛（MDA）、睾酮和总抗氧化能力（TAC）的血清水平。左尾附睾用于分析精子参数。p  < 0.05。睾丸体积、曲细精管体积、精母细胞数、圆形和长形精子细胞、生精指标、精子活力、计数、活力、尾长和 DSP 等精子参数、血清睾酮水平、TAC 和体重显着下降。地塞米松组与对照组相比。同时，与对照组相比，地塞米松组间质组织明显肿胀，MDA水平显着升高。上述参数在地塞米松+褪黑素组达到对照水平。褪黑激素可以补偿地塞米松对小鼠精子参数和曲细精管组织学的不良影响。