Abstract Repetitive DNA is an important component of eukaryotic genomes, accounting for more than 90% of the genome size of some species, including mobile elements and satellite DNA sequences. The aim of study was to characterize the genome of Hancornia speciosa Gomes using C-value genome size estimate and repetitive DNA sequences analysis. The genome size estimate was obtained by flow cytometry and the repetitive DNA sequences were accessed using graph-based clustering. Evolutionary relationships among species of Apocynaceae was obtained using reads of Catharanthus roseus L., Rhayza stricta Decne, and Asclepias syriaca L. from the NCBI and analyzed by graph-based clustering. The genome size estimates in two botanical varieties showed 2C-values ranging from 0.88 to 1.08 pg, indicating small genome size. Clusters representing repeats making up at least 0.01% of the genome revealed the proportion of repetitive DNA ranging from 19.87% (H. speciosa) to 51.674% (A. syriaca), of which the mobile elements were more abundant. Satellite DNA sequences were not found in H. speciosa and R. stricta, while at least one satellite was detected in C. roseus and A. syriaca, suggesting that the LTR retrotransposon Ty3/Gypsy/Chromovirus may have replaced the satellite DNA in H. speciosa and R. stricta.

中文翻译：

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使用低覆盖度测序鉴定 Hancornia spiosa Gomes（夹竹桃科：龙胆目）的基因组大小和重复 DNA 序列


摘要 重复DNA是真核基因组的重要组成部分，占某些物种基因组大小的90%以上，包括移动元件和卫星DNA序列。研究的目的是利用 C 值基因组大小估计和重复 DNA 序列分析来表征 Hancornia spiosa Gomes 的基因组。通过流式细胞术获得基因组大小估计值，并使用基于图形的聚类访问重复的 DNA 序列。使用来自 NCBI 的长春花 L.、Rhayza stricta Decne 和 Asclepias syriaca L. 的读数获得夹竹桃科物种之间的进化关系，并通过基于图形的聚类进行分析。两个植物品种的基因组大小估计显示 2C 值范围为 0.88 至 1.08 pg，表明基因组大小较小。代表基因组至少 0.01% 重复的簇揭示了重复 DNA 的比例范围从 19.87% (H. spiosa) 到 51.674% (A. syriaca)，其中移动元件更加丰富。在 H. spiosa 和 R. stricta 中未发现卫星 DNA 序列，而在 C. Roseus 和 A. syriaca 中至少检测到一个卫星序列，表明 LTR 逆转录转座子 Ty3/Gypsy/Chromovirus 可能取代了 H. spiosa 中的卫星 DNA。 spiosa 和 R. stricta。