BACKGROUND Non-human primates contribute to the spread of the yellow fever virus (YFV) and the establishment of transmission cycles in endemic areas. OBJECTIVE To describe the severe histopathological aspects of YFV infection, 10 squirrel monkeys were infected with YFV and blood, brain, liver, kidney, spleen, heart, lung, lymph node and stomach were collected at 1-7, 10, 20 and 30 days post-infection (dpi). METHODS Histopathological analysis and detection of the genome and viral antigens and neutralising antibodies were performed by RT-PCR, immunohistochemistry and neutralisation test, respectively. FINDINGS Only one animal died from the experimental infection. The genome and viral antigens were detected in all investigated organs (1-30 dpi) and the neutralising antibodies from seven to 30 dpi. The brain contained perivascular haemorrhage (6 dpi); in the liver, midzonal haemorrhage and lytic necrosis (6 dpi) were observed. The kidney had bleeding in the Bowman’s capsule and tubular necrosis (6 dpi). Pyknotic lymphocytes were observed in the spleen (1-20 dpi), the lung had haemorrhage (2-6 dpi), in the endocardium it contained nuclear pyknosis and necrosis (2-3 dpi) and the stomach contained blood in the lumen (6 dpi). MAIN FINDINGS Squirrel monkeys reliably reproduced the responses observed in human cases of yellow fever and, therefore, constitute an excellent experimental model for studies on the pathophysiology of the disease.

中文翻译：

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松鼠猴（Saimiri spp.）实验性黄热病病毒感染 I：尸检时器官的大体解剖学和组织病理学发现


背景技术非人灵长类动物有助于黄热病病毒(YFV)的传播和在流行地区建立传播周期。目的 为了描述 YFV 感染的严重组织病理学方面，10 只松鼠猴感染 YFV，并在 1-7、10、20 和 30 天收集血液、脑、肝、肾、脾、心、肺、淋巴结和胃。感染后 (dpi)。方法分别采用RT-PCR、免疫组化和中和试验进行基因组、病毒抗原和中和抗体的组织病理学分析和检测。结果 只有一只动物死于实验感染。在所有研究的器官（1-30 dpi）中检测到基因组和病毒抗原，并在 7 至 30 dpi 中检测到中和抗体。脑部有血管周围出血（6 dpi）；在肝脏中，观察到中带出血和溶解性坏死（6 dpi）。肾脏鲍曼囊出血和肾小管坏死（6 dpi）。脾脏中观察到淋巴细胞固缩（1-20 dpi），肺有出血（2-6 dpi），心内膜有核固缩和坏死（2-3 dpi），胃管腔中有血液（6 dpi）。主要发现松鼠猴可靠地再现了在人类黄热病病例中观察到的反应，因此构成了研究该疾病病理生理学的优秀实验模型。