IFI44L expression is regulated by IRF‐1 and HIV‐1,FEBS Open Bio

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IFI44L expression is regulated by IRF‐1 and HIV‐1
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-11-06 , DOI: 10.1002/2211-5463.13030
Yutong Li ₁ , Junshi Zhang ₁ , Chenchen Wang ₁ , Wentao Qiao ₁ , Yue Li _{1,

2} , Juan Tan ₁

Affiliation

Interferon (IFN)‐inducible 44 like (IFI44L) is an IFN‐stimulated gene (ISG), which is located on the same chromosome as the known antiviral ISG IFI44. Expression of IFI44L is induced by IFN and HIV‐1 infection. However, the mechanism by which IFN‐I induces IFI44L production has not yet been determined. In this study, we analyzed transcriptional regulation of IFI44L via cloning of the IFI44L promoter. We found that IFI44L has two IFN‐stimulated response elements (ISRE), which are necessary for the basal level of IFI44L transcription. IFN‐I and IFN‐II can activate the IFI44L promoter through one of the two ISREs. IFN regulatory factor (IRF)‐1 can activate transcription of IFI44L by binding to one of the ISREs. Additionally, co‐transfection of the IFI44L promoter with an HIV‐1 infectious clone or HIV‐1 infection activated IFI44L promoter transcription, but did not upregulate IFI44L expression via ISREs. These findings will help to understand the interaction between IFI44L and HIV‐1, and aid in elucidation of the role of IFI44L in the antiviral innate immune response.

中文翻译：

IFI44L 表达受 IRF-1 和 HIV-1 调节

干扰素 (IFN) 诱导型 44 样 (IFI44L) 是一种 IFN 刺激基因 (ISG)，与已知的抗病毒 ISG IFI44 位于同一染色体上。IFI44L 的表达由 IFN 和 HIV-1 感染诱导。然而，尚未确定 IFN-I 诱导 IFI44L 产生的机制。在这项研究中，我们通过克隆IFI44L启动子分析了 IFI44L 的转录调控。我们发现IFI44L有两个 IFN 刺激的反应元件 (ISRE)，它们是IFI44L转录的基础水平所必需的。IFN-I 和 IFN-II 可以通过两个 ISRE 之一激活IFI44L启动子。IFN调节因子（IRF）-1可以激活IFI44L的转录通过绑定到其中一个 ISRE。此外，IFI44L启动子与 HIV-1 感染性克隆或 HIV-1 感染的共转染可激活IFI44L启动子转录，但不会通过 ISRE 上调IFI44L表达。这些发现将有助于了解 IFI44L 和 HIV-1 之间的相互作用，并有助于阐明 IFI44L 在抗病毒先天免疫反应中的作用。

更新日期：2021-01-04

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