Regeneration and restoring the function of the myocardial‐infracted hearts have been one of the constant challenges in medicine. Recently, tissue engineering, using biocompatible substrates and stem cells, holds a real promise to solve these problems. Herein, poly(lactic‐co‐glycolic acid) (PLGA) nanofibers and platelet‐rich plasma (PRP) enriched PLGA nanofibers (PLGA‐PRP) were fabricated by electrospinning. Scanning electron microscopy (SEM) demonstrated that fiber diameters in PLGA scaffolds with and without PRP were in the range of 500 ± 280 nm and fibers were also bead free, smooth, in random orientation, and with interconnected pores. During culture of the human‐induced pluripotent stem cells (iPSCs) on the nanofibrous scaffold, further differentiation of the iPSCs to cardiomyocytes was detected in PLGA‐PRP nanofibers compared to the PLGA. This improvement in differentiation potential was evaluated at the morphological, molecular gene, and protein expression levels using SEM, real‐time reverse transcription‐polymerase chain reaction (RT‐PCR), and immunocytochemistry, respectively. The results obtained in this study highlighted the significance of natural growth factors present in the artificial scaffold applied in cardiac tissue engineering according to the improvements in cell‐biomaterial interactions. Taken together, our result indicated that PRP‐incorporated PLGA could be considered as a great potential candidate to use for engineering suitable myocardium replacement constructs.

中文翻译：

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富含血小板血浆的PLGA纳米纤维上诱导多能干细胞的高效心肌分化

心肌梗塞后心脏的再生和恢复功能一直是医学领域的持续挑战之一。最近，使用生物相容性基质和干细胞的组织工程技术有望真正解决这些问题。在此，通过电纺丝制造了聚乳酸-乙醇酸（PLGA）纳米纤维和富血小板血浆（PRP）富集的PLGA纳米纤维（PLGA-PRP）。扫描电子显微镜（SEM）表明，在有和没有PRP的PLGA支架中，纤维直径在500±280 nm范围内，并且纤维也无珠，光滑，无规取向并具有相互连接的孔。与PLGA相比，在纳米纤维支架上培养人诱导的多能干细胞（iPSC）期间，在PLGA-PRP纳米纤维中检测到iPSC进一步分化为心肌细胞。分别使用SEM，实时逆转录聚合酶链反应（RT-PCR）和免疫细胞化学方法在形态，分子基因和蛋白质表达水平上评估了分化潜能的提高。这项研究获得的结果强调了根据细胞-生物材料相互作用的改善，存在于心脏组织工程中的人工支架中存在的自然生长因子的重要性。两者合计，我们的结果表明掺入PRP的PLGA被认为是用于工程改造合适的心肌替代构建物的巨大潜力。和免疫细胞化学分别。这项研究获得的结果强调了根据细胞-生物材料相互作用的改善，存在于心脏组织工程中的人工支架中存在的自然生长因子的重要性。两者合计，我们的结果表明掺入PRP的PLGA被认为是用于工程改造合适的心肌替代构建物的巨大潜力。和免疫细胞化学分别。这项研究获得的结果强调了根据细胞-生物材料相互作用的改善，存在于心脏组织工程中的人工支架中存在的自然生长因子的重要性。两者合计，我们的结果表明掺入PRP的PLGA被认为是用于工程改造合适的心肌替代构建物的巨大潜力。