Sequence analysis of 43‐year old samples of Plantago lanceolata show that Plantain virus X is synonymous with Actinidia virus X and is widely distributed,Plant Pathology

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Sequence analysis of 43‐year old samples of Plantago lanceolata show that Plantain virus X is synonymous with Actinidia virus X and is widely distributed
Plant Pathology ( IF 2.3 ) Pub Date : 2020-11-07 , DOI: 10.1111/ppa.13310
John Hammond ₁ , Ian P. Adams _{2,

3} , Aimee R. Fowkes ₂ , Sam McGreig ₂ , Marleen Botermans ₄ , Joanieke J. A. Oorspronk ₄ , Marcel Westenberg ₄ , Martin Verbeek ₅ , Annette M. Dullemans ₅ , Christina C. M. M. Stijger ₅ , Arnaud G. Blouin ₆ , Sebastien Massart ₆ , Kris De Jonghe ₇ , Maaike Heyneman ₇ , John A. Walsh ₈ , Adrian Fox ₂

Affiliation

Plantain virus X was first recognized by the ICTV as a species in the genus Potexvirus in 1982. However, because no sequence was available for plantain virus X (PlVX), abolishing the species was proposed to the Flexiviridae working group of the ICTV in 2015. This initiated efforts to sequence the original isolates from Plantago lanceolata samples. Here we report the full‐genome sequencing of two original isolates of PlVX, which demonstrate that the virus is synonymous to Actinidia virus X, a species previously reported from kiwifruit (Actinidia sp.) and blackcurrant (Ribes nigrum). PlVX was previously noted to be widespread in the UK in P. lanceolata. This report additionally presents novel data on the distribution and diversity of PlVX, collected at the same site as the original UK isolates, and from three independent surveys, two in the Netherlands and one in Belgium. This study also includes two new host records for PlVX, Browallia americana and Capsicum annuum (sweet pepper), indicating the virus is more widespread and infects a broader range of hosts than previously reported. This stresses the importance of surveys of noncultivated species to gain insight into viral distribution and host range. This study also demonstrates the value of generating sequence data for isolates retained in virus collections. Additionally, it demonstrates the potential value in prepublication data sharing for giving context to virus detections such as the four independent studies here which, when combined, give greater clarity to the identity, diversity, distribution, and host range of plantain virus X.

中文翻译：

对43岁的车前草样品的序列分析表明，车前草病毒X与猕猴桃病毒X是同义词，并且广泛分布

车前病毒X于1982年首次被ICTV识别为Potex病毒属中的一个物种。但是，由于没有针对车前病毒X（PlVX）的序列，因此在2015年向ICTV的Flexiviridae工作组提议取消该物种。这启动了对从车前草样品中分离出的原始菌株进行测序的工作。在这里，我们报告了PlVX的两个原始分离株的全基因组测序，这表明该病毒与猕猴桃X病毒同义，该猕猴桃以前是从猕猴桃（Actinidia sp。）和黑加仑（Ribes nigrum）中报道的。先前已指出PlVX在英国的P中很普遍。披针形。本报告还提供了与PlVX的分布和多样性有关的新颖数据，这些数据与原始的英国分离株在同一地点收集，来自三项独立的调查，其中两项在荷兰，一项在比利时。这项研究还包括两个新的宿主病毒记录，分别为PlVX，美洲Browallia和辣椒。（甜椒），表明该病毒比以前报道的更广泛传播，并感染更广泛的宿主。这强调了非栽培物种调查对了解病毒分布和寄主范围的重要性。这项研究还证明了为保留在病毒收集物中的分离株生成序列数据的价值。此外，它还展示了在发布前数据共享中为病毒检测提供背景的潜在价值，如此处的四项独立研究相结合，可以更清晰地识别车前草病毒X的身份，多样性，分布和寄主范围。

更新日期：2021-01-06

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