Research in the field of neurobiology and neurochemistry has seen a rapid expansion in the last several years due to advances in technologies and instrumentation, facilitating the detection of biomolecules critical to the complex signaling of neurons. Part of this growth has been due to the development and implementation of high-resolution Fourier transform (FT) mass spectrometry (MS), as is offered by FT ion cyclotron resonance (FTICR) and Orbitrap mass analyzers, which improves the accuracy of measurements and helps resolve the complex biological mixtures often analyzed in the nervous system. The coupling of matrix-assisted laser desorption/ionization (MALDI) with high-resolution MS has drastically expanded the information that can be obtained with these complex samples. This review discusses notable technical developments in MALDI-FTICR and MALDI-Orbitrap platforms and their applications toward molecules in the nervous system, including sequence elucidation and profiling with de novo sequencing, analysis of post-translational modifications, in situ analysis, key advances in sample preparation and handling, quantitation, and imaging. Notable novel applications are also discussed to highlight key developments critical to advancing our understanding of neurobiology and providing insight into the exciting future of this field. © 2020 John Wiley & Sons Ltd. Mass Spec Rev

中文翻译：

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用于神经生物学的高分辨率 MALDI 质谱法的进展

由于技术和仪器的进步，神经生物学和神经化学领域的研究在过去几年中迅速扩大，促进了对神经元复杂信号传导至关重要的生物分子的检测。这种增长的部分原因是高分辨率傅里叶变换 (FT) 质谱 (MS) 的开发和实施，如 FT 离子回旋共振 (FTICR) 和 Orbitrap 质量分析仪所提供的，它提高了测量的准确性和有助于解决神经系统中经常分析的复杂生物混合物。基质辅助激光解吸/电离 (MALDI) 与高分辨率 MS 的结合极大地扩展了这些复杂样品可以获得的信息。从头测序、翻译后修饰分析、原位分析、样品制备和处理、定量和成像方面的关键进展。还讨论了值得注意的新应用，以突出对促进我们对神经生物学的理解和深入了解该领域令人兴奋的未来至关重要的关键发展。© 2020 John Wiley & Sons Ltd. 质谱修订版