Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-β fibrils,Magnetic Resonance in Chemistry

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Deuterium solid-state NMR quadrupolar order rotating frame relaxation with applications to amyloid-β fibrils
Magnetic Resonance in Chemistry ( IF 1.9 ) Pub Date : 2020-11-08 , DOI: 10.1002/mrc.5114
Liliya Vugmeyster ₁ , Dmitry Ostrovsky ₂

Affiliation

We describe a new method for measuring molecular dynamics based on the deuterium solid-state nuclear magnetic resonance (NMR) quadrupolar order rotating frame relaxation rate R_1ρ,Q under static conditions. The observed quadrupolar order coherence is created using the broad-band Jeener–Broekaert excitation and is locked with a weak radio frequency (RF) field. We describe the experimental and theoretical approaches and show applications to a selectively deuterated valine side chain of the phosphorylated amyloid-β (1–40) fibrils phosphorylated at the serine-8 position. The R_1ρ,Q rate is sensitive to the rotameric exchange mode. For biological samples, the low spin-lock field in the 5- to 10-kHz range has the advantage of avoiding sample heating and dehydration. Thus, it provides an alternative to approaches based on single-quantum coherence, which require larger spin-lock fields.

中文翻译：

氘固态核磁共振四极级旋转框架弛豫及其在淀粉样蛋白-β原纤维中的应用

我们描述了一种在静态条件下基于氘固态核磁共振（NMR）四极级旋转框架弛豫率R _{1 ρ ,Q}测量分子动力学的新方法。观察到的四极阶相干性是使用宽带 Jeener-Broekaert 激励创建的，并用弱射频 (RF) 场锁定。我们描述了实验和理论方法，并展示了在丝氨酸 8 位磷酸化的磷酸化淀粉样蛋白-β (1-40) 原纤维的选择性氘代缬氨酸侧链上的应用。 R _{1 ρ ,Q}速率对旋转异构体交换模式敏感。对于生物样品，5 至 10 kHz 范围内的低自旋锁场具有避免样品加热和脱水的优点。因此，它为基于单量子相干性的方法提供了一种替代方案，因为单量子相干性需要更大的自旋锁场。

更新日期：2020-11-08

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