3D Printing of Bone‐Mimetic Scaffold Composed of Gelatin/β‐Tri‐Calcium Phosphate for Bone Tissue Engineering,Macromolecular Bioscience

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3D Printing of Bone‐Mimetic Scaffold Composed of Gelatin/β‐Tri‐Calcium Phosphate for Bone Tissue Engineering
Macromolecular Bioscience ( IF 4.4 ) Pub Date : 2020-11-08 , DOI: 10.1002/mabi.202000256
Jae Eun Jeong _{1,

2} , Shin Young Park ₃ , Ji Youn Shin ₂ , Ji Min Seok ₁ , June Ho Byun ₄ , Se Heang Oh ₅ , Wan Doo Kim ₁ , Jun Hee Lee ₁ , Won Ho Park ₂ , Su A Park ₁

Affiliation

3D printed scaffolds composed of gelatin and β‐tri‐calcium phosphate (β‐TCP) as a biomimetic bone material are fabricated, thereby providing an environment appropriate for bone regeneration. The Ca²⁺ in β‐TCP and COO⁻ in gelatin form a stable electrostatic interaction, and the composite scaffold shows suitable rheological properties for bioprinting. The gelatin/β‐TCP scaffold is crosslinked with glutaraldehyde vapor and unreacted aldehyde groups which can cause toxicity to cells is removed by a glycine washing. The stable binding of the hydrogel is revealed as a result of FTIR and degradation rate. It is confirmed that the composite scaffold has compressive strength similar to that of cancellous bone and 60 wt% β‐TCP groups containing 40 wt% gelatin have good cellular activity with preosteoblasts. Also, in the animal experiments, the gelatin/β‐TCP scaffold confirms to induce bone formation without any inflammatory responses. This study suggests that these fabricated scaffolds can serve as a potential bone substitute for bone regeneration.

中文翻译：

用于骨组织工程的由明胶/β-磷酸三钙组成的骨模拟支架的 3D 打印

制造由明胶和β-磷酸三钙（β-TCP）组成的3D打印支架作为仿生骨材料，从而提供适合骨再生的环境。β-TCP和COO中的Ca ²⁺^-在明胶中形成稳定的静电相互作用，复合支架显示出适合生物打印的流变学特性。明胶/β-TCP 支架与戊二醛蒸汽交联，并通过甘氨酸洗涤去除对细胞造成毒性的未反应醛基。FTIR 和降解率显示了水凝胶的稳定结合。证实复合支架具有与松质骨相似的抗压强度，含有 40 wt% 明胶的 60 wt% β-TCP 基团对前成骨细胞具有良好的细胞活性。此外，在动物实验中，明胶/β-TCP 支架证实可诱导骨形成而没有任何炎症反应。这项研究表明，这些制造的支架可以作为骨再生的潜在骨替代品。

更新日期：2020-12-15

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