Association between temperament related traits and single nucleotide polymorphisms in the serotonin and oxytocin systems in Merino sheep,Genes, Brain and Behavior

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Association between temperament related traits and single nucleotide polymorphisms in the serotonin and oxytocin systems in Merino sheep
Genes, Brain and Behavior ( IF 2.4 ) Pub Date : 2020-11-08 , DOI: 10.1111/gbb.12714
Luoyang Ding _{1,

2} , Shane K Maloney ₃ , Mengzhi Wang ₂ , Jennifer Rodger ₄ , Lianmin Chen ₅ , Dominique Blache ₁

Affiliation

Animal temperament is defined as the consistent behavioral and physiological differences that are seen between individuals in response to the same stressor. Neurotransmitter systems, like serotonin and oxytocin in the central nervous system, underlie variation in behavioral traits in humans and other animals. Variations like single nucleotide polymorphisms (SNPs) in the genes for tryptophan 5‐hydroxylase (TPH2), the serotonin transporter (SLC6A4), the serotonin receptor (HTR2A), and the oxytocin receptor (OXTR) are associated with behavioral phenotype in humans. Thus, the objective of this study was to identify SNPs in those genes and to test if those variations are associated with the temperament in Merino sheep. Using ewes from the University of Western Australia temperament flock, which has been selected on emotional reactivity for more than 20 generations, eight SNPs (rs107856757, rs107856818, rs107856856 and rs107857156 in TPH2, rs20917091 in SLC6A4, rs17196799 and rs17193181 in HTR2A, and rs17664565 in OXTR) were found to be distributed differently between calm and nervous sheep. These eight SNPs were then genotyped in 260 sheep from a flock that has never been selected on emotional reactivity, followed by the estimation of the behavioral traits of those 260 sheep using an arena test and an isolation box test. We found that several SNPs in TPH2 (rs107856757, rs107856818, rs107856856 and rs107857156) were in strong linkage disequilibrium, and all were associated with behavioral phenotype in the nonselected sheep. Similarly, rs17196799 in HTR2A was also associated with the behavioral phenotype.

中文翻译：

美利奴羊5-羟色胺和催产素系统中气质相关性状与单核苷酸多态性的关联

动物气质被定义为个体之间在应对相同压力源时所看到的一致的行为和生理差异。神经递质系统，如中枢神经系统中的血清素和催产素，是人类和其他动物行为特征变异的基础。色氨酸 5-羟化酶 ( TPH2 )、5-羟色胺转运蛋白 ( SLC6A4 )、5-羟色胺受体 ( HTR2A ) 和催产素受体 ( OXTR ) 基因中的单核苷酸多态性 (SNP) 变异) 与人类的行为表型有关。因此，本研究的目的是鉴定这些基因中的 SNP，并测试这些变异是否与美利奴羊的性情有关。使用来自西澳大利亚大学气质羊群的母羊，其情绪反应性已超过 20 代，8 个 SNP（TPH2中的 rs107856757、rs107856818、rs107856856 和 rs107857156 、SLC6A4 中的rs20917091、rs17196799和rs17193181 OXTR) 被发现在平静和紧张的绵羊之间分布不同。然后，对从未根据情绪反应选择过的羊群中的 260 只绵羊进行这 8 个 SNP 的基因分型，然后使用竞技场测试和隔离盒测试估计这 260 只绵羊的行为特征。我们发现TPH2中的几个 SNP （rs107856757、rs107856818、rs107856856 和 rs107857156）处于强连锁不平衡状态，并且都与未选择绵羊的行为表型相关。同样，HTR2A中的 rs17196799 也与行为表型相关。

更新日期：2020-11-08

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