Borneol is a commonly used flavouring substance in traditional Chinese medicine, which possesses several pharmacological activities including analgesic, antiinflammatory, and antioxidant properties. The aim of this study was to investigate the effects of borneol on cerulein-induced acute pancreatitis (AP) model. Swiss albino mice were pretreated with borneol (100 and 300 mg/kg) daily for 7 days, before six consecutive injections of cerulein (50 μg/kg/hr, intraperitoneally). The protective effect of borneol was studied by biochemical, enzyme linked immunosorbent assay, histological, immunoblotting, and immunohistochemical analysis. Oral administration of borneol significantly attenuated pancreatic damage by reducing amylase, lipase levels and histological changes. Borneol attenuated cerulein-induced oxidative-nitrosative stress by decreasing malondialdehyde, nitrite levels, and elevating reduced glutathione levels. Pancreatic inflammation was ameliorated by inhibiting myeloperoxidase activity and pro-inflammatory cytokine (Interleukins and TNF-α) levels. Furthermore, borneol administration significantly increased nuclear factor E2-related factor 2 (Nrf2), superoxide dismutase (SOD1) expression and reduced phospho-NF-κB p65 expression. Treatment with borneol significantly inhibited TNF-α, IL-1β, IL-6, and inducible nitric oxide synthase expression in cerulein-induced AP mouse model. Together, these results indicate that borneol which is currently used as US-FDA approved food adjuvant has the potential to attenuate cerulein-induced AP possibly by reducing the oxidative damage and pancreatic inflammation by modulating Nrf2/NF-κB pathway.

中文翻译：

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冰片可防止急性胰腺炎小鼠模型中蔚蓝素诱导的氧化应激和炎症

冰片是中药中常用的调味物质，具有多种药理活性，包括镇痛、抗炎和抗氧化特性。本研究的目的是研究冰片对天蓝蛋白诱导的急性胰腺炎 (AP) 模型的影响。瑞士白化小鼠每天用冰片（100 和 300 毫克/公斤）预处理 7 天，然后连续六次注射蔚蓝素（50 微克/公斤/小时，腹膜内）。通过生化、酶联免疫吸附试验、组织学、免疫印迹和免疫组织化学分析研究冰片的保护作用。口服冰片通过降低淀粉酶、脂肪酶水平和组织学变化显着减轻胰腺损伤。冰片通过降低丙二醛、亚硝酸盐水平和提高还原型谷胱甘肽水平来减轻蔚蓝素诱导的氧化亚硝化应激。通过抑制髓过氧化物酶活性和促炎细胞因子（白细胞介素和 TNF-α）水平来改善胰腺炎症。此外，冰片给药显着增加了核因子 E2 相关因子 2 (Nrf2)、超氧化物歧化酶 (SOD1) 的表达并降低了磷酸化-NF-κB p65 的表达。冰片处理显着抑制了雨蛙素诱导的 AP 小鼠模型中 TNF-α、IL-1β、IL-6 和诱导型一氧化氮合酶的表达。一起，