Cell‐derived microvesicles (MVs, also known as microparticles, MPs) are known as important biomarkers of various diseases including cardiovascular diseases, infectious diseases, and cancer. Development of techniques for blood cell removal and MV isolation is required for downstream analyses in biological and clinical applications. A sheathless, label‐free, viscoelastic microfluidic device is proposed for the separation of submicrometer platelet‐derived MPs (PDMPs) from whole blood. Flow rate and channel length dependent performance of the device is evaluated using 2‐µm particles as analogs to platelets and hematocrit (Hct) dependent flow characteristics of red blood cells (RBCs) are observed. In addition, the removal rate of nanoparticles of sizes 300, 500, 700, and 1000 nm is evaluated, and finally, the device is applied to examine the recovery of PDMPs from diluted whole blood sample. At Q = 200 µL min⁻¹ in a 15‐mm long microchannel, RBCs at 5% Hct and platelets are removed to the central outlet without platelet activation, while PDMPs are successfully isolated to the side outlets within ≈2 min. The PDMPs collected from the device are analyzed by flow cytometry. The device exhibits ≈4.8‐fold enhanced isolation performance compared to the centrifugation method.

中文翻译：

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使用粘弹性流体高通量分离全血成分中的微囊泡

细胞源性微囊泡（MVs，也称为微粒，MPs）被称为各种疾病的重要生物标志物，包括心血管疾病，传染病和癌症。对于生物学和临床应用中的下游分析，需要开发用于去除血细胞和分离MV的技术。提出了一种无鞘，无标签的粘弹性微流体装置，用于从全血中分离亚微米血小板衍生的MP（PDMP）。使用2 µm颗粒作为血小板的类似物来评估设备的流速和通道长度相关性能，并观察到取决于血细胞比容（Hct）的红细胞（RBC）流动特性。另外，评估尺寸为300、500、700和1000 nm的纳米颗粒的去除率，最后，该设备用于检查稀释的全血样品中PDMP的回收率。在Q  = 200 µL min ^-1，位于15 mm长的微通道中，Rcts在5％Hct时被清除，而血小板没有被激活就被转移到中央出口，而PDMPs在≈2min内被成功地分离到了侧面出口。通过流式细胞仪分析从设备收集的PDMP。与离心法相比，该器件的隔离性能提高了约4.8倍。