Patient‐Specific Bone Particles Bioprinting for Bone Tissue Engineering,Advanced Healthcare Materials

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Patient‐Specific Bone Particles Bioprinting for Bone Tissue Engineering
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-11-09 , DOI: 10.1002/adhm.202001323
Greeshma Ratheesh ₁ , Cedryck Vaquette ₂ , Yin Xiao _{1,

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Affiliation

Although bioinks with both high printability and shape fidelity while maintaining high cell viability are developed, the biofunctionality of the resulting bioprinted construct is often overlooked. To address this, a methacrylated gelatin (GelMA)‐based bioink biofunctionalized with bone particles (BPs) is developed as a personalized treatment strategy for bone regeneration. The bioink consists of incorporating BPs of various sizes (0–500 µm) in GelMA at various concentrations (ranging from 5 to 15% w/v). The printability of the bioink is systematically investigated and it is demonstrated that a 15% w/v BP‐loading results in high print quality for 10% and 12.5% GelMA concentrations. Rheological evaluation reveals a strong shear thinning behavior essential for printing and a high gel strength in bioink with 15% w/v 0–500 µm BPs for both GelMA concentrations. In addition, the printability of the bioink and the metabolic activity of the resulting scaffolds are dependent on both the concentration of hydrogel and size of the BPs. Importantly, the cells initially contained in the BPs are able to migrate and colonize the bioprinted scaffold while maintaining their capacity to express early osteogenic markers. This study demonstrates the feasibility of bioprinted viable BPs and may have some potential for chairside clinical translation.

中文翻译：

用于骨组织工程的患者特异性骨颗粒生物打印

尽管开发了具有高可打印性和形状保真度同时保持高细胞活力的生物墨水，但所得生物打印结构的生物功能性常常被忽视。为了解决这个问题，开发了一种基于甲基丙烯酸明胶（GelMA）的生物墨水，并用骨颗粒（BP）进行生物功能化，作为骨再生的个性化治疗策略。该生物墨水由不同浓度（5% 至 15% w/v）的 GelMA 中掺入不同尺寸（0-500 µm）的 BP 组成。对生物墨水的可打印性进行了系统研究，结果表明，15% w/v BP 负载量可在 10% 和 12.5% GelMA 浓度下实现高打印质量。流变学评估表明，对于两种 GelMA 浓度，印刷所必需的强剪切稀化行为以及生物墨水中具有 15% w/v 0–500 µm BP 的高凝胶强度。此外，生物墨水的可打印性和所得支架的代谢活性取决于水凝胶的浓度和 BP 的大小。重要的是，最初包含在 BP 中的细胞能够迁移并定植于生物打印的支架上，同时保持其表达早期成骨标志物的能力。这项研究证明了生物打印可行的 BP 的可行性，并且可能具有一定的椅旁临床转化潜力。

更新日期：2020-12-02

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