Abstract Dielectrophoresis (DEP) is known as an attractive and frugal technique to manipulate biological particles in microfluidics. This study presents the advanced solution strategy of a DEP-based microfluidic channel for focusing and separating cancerous cells in continuous flow. Theoretical calculations were carried out to define the favorable parameters in the electric field operation of the microchip. A simulation model was also used to explore the performance of the design in the isolation of circulating tumor cells (CTCs). It revealed that the optimal conditions of the device are suitable to effectively separate CTCs from red blood cells (RBCs) within the channel structure, with a high flow rate of 1.5 μL/min, and an electric amplitude as low as 10 Vpp, at the frequency of 1 kHz. The proposed method has shown potential as a simple, easy-to-operate, and low-cost approach enable to enhance the diagnosis systems for cancer detection at early stages.

中文翻译：

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基于介电泳的微流体通道中连续细胞分离的应用电场分析和数值研究

摘要介电泳 (DEP) 被认为是一种在微流体中操纵生物颗粒的有吸引力且省钱的技术。本研究提出了基于 DEP 的微流体通道的先进解决方案策略，用于在连续流动中聚焦和分离癌细胞。进行理论计算以定义微芯片电场操作中的有利参数。还使用模拟模型来探索该设计在分离循环肿瘤细胞 (CTC) 方面的性能。结果表明，该装置的最佳条件适合在通道结构内有效地将 CTC 与红细胞 (RBC) 分离，高流速为 1.5 μL/min，电振幅低至 10 Vpp，在频率为 1 kHz。所提出的方法已显示出作为一种简单、