The aim of this study was to estimate the diversity and prevalence of both groups of Brucella canis 1 and 2 with and without deletion respectively in different areas of Argentina. A total of 104 bacterial cultures were typed as B. canis strains using the classical biotyping method. Two PCR assays were performed to confirm that all isolates were B. canis and not Brucella suis. The differentiation between groups 1 and 2 was achieved using another PCR assay and the diversity of B. canis isolates was assessed with four MLVA_16 markers. All strains belonged to Group 2. Bruce 09 marker (MLVA_16 assay) showed the greatest diversity. Only Group 2 of B. canis was identified among the strains evaluated. The markers chosen from the MLVA_16 allowed us to detect genetic diversity among the strains of B. canis studied.

本研究的目的是估计阿根廷不同地区分别有缺失和不缺失的犬布鲁氏菌1 和 2群的多样性和流行率。使用经典生物分型方法将总共​​ 104 种细菌培养物分型为犬双歧杆菌菌株。进行了两次 PCR 测定以确认所有分离株均为犬双歧杆菌而非猪布鲁氏菌。第 1 组和第 2 组之间的区别是使用另一个 PCR 测定实现的，并且犬双歧杆菌分离株的多样性使用四个 MLVA_16 标记进行评估。所有菌株都属于第 2 组。Bruce 09 标记（MLVA_16 测定）显示出最大的多样性。只有B. canis 的第 2 组在评估的菌株中鉴定出。从 MLVA_16 中选择的标记使我们能够检测所研究的犬双歧杆菌菌株之间的遗传多样性。