An entomopathogenic fungus was isolated from an infected larva of Conogethes punctiferalis (Guenée) (Crambidae: Lepidoptera), a highly polyphagous pest recorded from more than 120 plants and widely distributed in Asia and Oceanic countries. The fungus was identified as Metarhizium pingshaense Q.T. Chen & H.L. Guo (Ascomycota: Hypocreales) based on morphological characteristics and molecular studies. Scanning electron microscopic studies were conducted to study the infection of C. punctiferalis by M. pingshaense. Bioassay studies with purified conidial suspension proved that the isolate was highly virulent to C. punctiferalis, causing more than 86 % mortality to fifth instar larvae at 1 × 10⁸ spores/mL, under laboratory conditions. The median lethal concentration (LC₅₀) of the fungus against late instar larvae was 9.1 × 10⁵ conidia/mL and the median survival time (MST) of late instar larvae tested at the doses of 1 × 10⁸ and 1 × 10⁷ conidia/mL were 4.7 and 6.4 days, respectively. The optimal temperature for fungal growth and sporulation was found to be 25 ± 1 °C. This is the first report of M. pingshaense naturally infecting C. punctiferalis. Isolation of a highly virulent strain of this fungus holds promise towards development of a potential mycoinsecticide against this pest.

从被感染的中华绒螯蟹（Guenée）（Crambidae：鳞翅目）的幼虫中分离出一种病原性真菌，该幼虫是一种高度多食性的害虫，记录于120多种植物中，广泛分布于亚洲和海洋国家。根据形态学特征和分子研究，该真菌被鉴定为平顶生真菌QT Chen和HL Guo（子囊菌：Hypocreales）。扫描电子显微镜研究方法，研究感染的C.螟通过中号。平山。用纯化的分生孢子悬浮液进行的生物测定研究证明，该分离物对点状梭菌具有高毒性。在实验室条件下，以1×10 ⁸孢子/ mL引起五龄幼虫的死亡率超过86％。真菌对晚8幼虫的致死浓度（LC ₅₀）为9.1×10 ⁵分生孢子/ mL，在1×10 ⁸和1×10 ⁷分生孢子剂量下测试的晚star幼虫的中位存活时间（MST）/ mL分别为4.7天和6.4天。真菌生长和孢子形成的最佳温度为25±1°C。这是M的第一个报告。pingshaense自然感染C.螟。分离出这种真菌的高毒力菌株有望开发出针对这种害虫的潜在杀真菌剂。