It has been previously shown that magnetite (Fe₃O₄) nanoparticles stimulate the anaerobic digestion process in several anaerobic reactors. Here we evaluate the effect of magnetite nanoparticles on the efficiency of anaerobic digestion of distillers grains with solubles and sugar beet pulp in mesophilic batch experiments. The addition of magnetite nanopowder had a positive effect on the anaerobic digestion process. CH₄ was produced faster in the presence of 50 mg of Fe₃O₄ per 1 g of added total solids than from treatments without addition of Fe₃O₄. These results demonstrate that the addition of magnetite enhances the methanogenic decomposition of organic acids. Microbial community structure and dynamics were investigated based on bacterial and archaeal 16S rRNA genes, as well as mcrA genes encoding the methyl-CoM reductase. Depending on the reactor, Bacteroides, midas_1138, Petrimonas, unclassified Rikenellaceae (class Bacteroidia), Ruminiclostridium, Proteiniclasticum, Herbinix, and Intestinibacter (class Clostridia) were the main representatives of the bacterial communities. The archaeal communities in well-performed anaerobic reactors were mainly represented by representatives of the genera Methanosarcina and Methanobacterium. Based on our findings, Fe₃O₄ nanoparticles, when used properly, will improve biomethane production.

先前已经表明，磁铁矿（Fe ₃ O ₄）纳米颗粒在几个厌氧反应器中刺激了厌氧消化过程。在这里，我们在中温分批实验中评估了磁铁矿纳米颗粒对酒糟和甜菜粕中厌氧消化酒糟的效率。磁铁矿纳米粉的添加对厌氧消化过程具有积极作用。在每1克添加的总固体中有50毫克的Fe ₃ O ₄的情况下，与未添加Fe ₃ O _4的处理相比，生成CH _4的速度更快。这些结果表明，磁铁矿的添加增强了有机酸的产甲烷分解。基于细菌和古细菌16S rRNA基因，以及编码甲基辅酶M还原酶的mcrA基因，研究了微生物群落结构和动力学。根据反应器，拟杆菌属，midas_1138，Petrimonas，未分类Rikenellaceae（类Bacteroidia），Ruminiclostridium，Proteiniclasticum，Herbinix，和Intestinibacter（类梭菌）是细菌群落的主要代表。表现良好的厌氧反应器中的古细菌群落主要以甲烷菌和甲烷菌属为代表。根据我们的发现，如果正确使用Fe ₃ O ₄纳米颗粒，将会提高生物甲烷的产生。