Abstract Soil waterlogging negatively impacts plants due to reduced oxygen availability in the rhizosphere. Here we selected two Coffea canephora clones with contrasting tolerance to drought and oxidative stress to test whether they would also exhibit contrasting tolerance to waterlogging and post-waterlogging reoxygenation. Plants were exposed to six days of waterlogging followed by 40 days of reoxygenation. During waterlogging, both clones exhibited lower leaf gas exchange rate, electron transport rate and photochemical quenching as well as increased non-photochemical quenching; overall, these changes were stronger in the drought tolerant (DT) clones over the drought sentitive (DS) ones. Photosynthetic limitations were essentially linked to diffusional constraints regardless of clone. In both clones, declines in stomatal conductance were not associated with foliar ABA or ethylene levels, however stomatal conductance correlated with the plant hydraulic conductance. After reoxygenation, DT plants mostly recovered leaf function and plant mortality was less than 10%. Conversely, DS plants displayed very low water potentials after reoxygenation, which was accompanied by photosynthetic and membrane damage and 40% plant mortality. Our findings confirmed that the DT clone is less impaired by soil waterlogging and reoxygenation than the DS one. The differences between clones were more evident during the reoxygenation than during waterlogging, likely due to the drought experienced by the DS but not DT plants. Collectively, this information leads us to a common path to find new coffee genotypes with increased tolerance to both drought and waterlogging aiming at increased coffee sustainability under an ongoing climate changing scenario.

中文翻译：

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耐旱咖啡植物对涝渍和涝渍后复氧表现出更高的耐受性

摘要 由于根际氧气供应量减少，土壤涝渍会对植物产生负面影响。在这里，我们选择了两个对干旱和氧化胁迫具有对比耐受性的 Coffea canephora 克隆，以测试它们是否也表现出对涝渍和涝渍后复氧的对比耐受性。将植物暴露于 6 天的涝渍，然后是 40 天的再充氧。淹水期间，两个无性系均表现出较低的叶片气体交换率、电子传递率和光化学猝灭，以及增加的非光化学猝灭；总体而言，这些变化在耐旱 (DT) 克隆中比对干旱敏感 (DS) 克隆更强。无论克隆如何，光合作用限制基本上都与扩散限制有关。在两个克隆中，气孔导度的下降与叶面 ABA 或乙烯水平无关，但气孔导度与植物水力导度相关。复氧后，DT植株叶功能基本恢复，植株死亡率低于10%。相反，DS 植物在复氧后表现出非常低的水势，伴随着光合作用和膜损伤以及 40% 的植物死亡率。我们的研究结果证实，与 DS 相比，DT 克隆受土壤涝渍和再充氧的影响较小。在复氧期间克隆之间的差异比在淹水期间更明显，这可能是由于 DS 而不是 DT 植物经历了干旱。总的来说，