In this study, the production and compositional analysis of exopolysaccharides produced by Bacillus cereus KMS3-1 grown in metal amended conditions were investigated. In addition, the metal adsorption efficacy of exopolysaccharides (EPS) produced by KMS3-1 strain was evaluated in a batch mode. Increased production of exopolysaccharides by KMS3-1 strain was observed while growing under metal amended conditions (100 mg/L) and also, the yield was in the order of Pb(II)>Cu(II)>Cd(II)>Control. Characterization of EPS using FT-IR, XRD, and SEM analysis revealed that the EPS can interact with metal ions through their functional groups (O‒H, CH, C˭O, C‒O, and C‒C˭O) and assist the detoxification process. Further, equilibrium results were fitted with the Langmuir model and notably, the maximum adsorption capacity (Q_max) of EPS for Cd(II), Cu(II), and Pb(II) found to be 54.05, 71.42, and 78.74 mg/g, respectively. To the best of our knowledge, EPS demonstrating proficient metal adsorption was substantiated by XRD analysis in this study. Owing to good adsorbing nature, the exopolysaccharides could be used as chelating substances for wastewater treatment.

在这项研究中，研究了在金属修饰条件下生长的蜡状芽孢杆菌KMS3-1产生的胞外多糖的组成和组成分析。另外，以分批方式评价了由KMS3-1菌株产生的胞外多糖（EPS）对金属的吸附效率。在金属修饰条件下（100 mg / L）生长时，观察到KMS3-1菌株胞外多糖的产量增加，并且产量依次为Pb（II）> Cu（II）> Cd（II）>对照。使用FT-IR，XRD和SEM分析EPS的特征表明EPS可以通过其官能团（O‒H，CH，C˭O，C‒O和C‒C˭O）与金属离子发生相互作用并起到辅助作用排毒过程。此外，平衡结果符合Langmuir模型，特别是最大吸附容量（QCd（II），Cu（II）和Pb（II）的EPS_最大值分别为54.05、71.42和78.74 mg / g。据我们所知，本研究中的XRD分析证实了EPS具有足够的金属吸附能力。由于具有良好的吸附性能，胞外多糖可用作废水处理中的螯合物质。