Multi-step one-pot chemoenzymatic cascade reactions (mo-CECRs) bridging chemo- and bio-catalysis can not only accomplish chemical transformations with minimized purification steps, low cost and improved stereochemical control, but also enhance reactivity and outcomes through the cooperative effect of multiple catalysts. Despite the attractiveness of the concept which meets the principles of green and sustainable chemistry, the establishment of such catalytic networks faces important challenges due to the incompatibilities between different catalytic disciplines. The purpose of this review is to chart progress on mo-CECRs, including temporal compartmentalization, spatial compartmentalization and chemo-bio nanoreactors, with an emphasis on platform methods and technologies involved. The perspectives on future challenges and strategies in this burgeoning research are declared. This review may provide guidance for the design of new and robust mo-CECRs.

跨化学反应和生物催化的多步一锅化学酶联反应（mo -CECR）不仅可以以最少的纯化步骤，低成本和改进的立体化学控制来完成化学转化，而且还可以通过多种催化剂。尽管符合绿色和可持续化学原理的概念具有吸引力，但由于不同催化学科之间的不相容性，建立这样的催化网络仍面临重要挑战。这篇评论的目的是为了绘制关于Mo的进度图-CECR，包括时间分隔，空间分隔和化学生物纳米反应器，重点在于所涉及的平台方法和技术。声明了这项新兴研究中有关未来挑战和策略的观点。这篇综述可以为新的和强大的mo- CECR的设计提供指导。