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Low-frequency, Green Sonoextraction of Antioxidants from Tree Barks of Hungarian Woodlands for Potential Food Applications
Chemical Engineering and Processing: Process Intensification ( IF 3.8 ) Pub Date : 2020-11-07 , DOI: 10.1016/j.cep.2020.108221
Charu Agarwal , Tamás Hofmann , Eszter Visi-Rajczi , Zoltán Pásztory

The present work evaluates and compares the antioxidant capacities of bioactive constituents in the barks of ten common wood species from Hungary (Quercus rubra, Prunus serotina, Quercus robur, Betula pendula, Fraxinus excelsior, Robinia pseudoacacia, Carpinus betulus, Picea abies, Alnus glutinosa, Pinus sylvestris). Low-frequency ultrasound was used for intensification of extraction from the bark to obtain extracts rich in polyphenolic antioxidants with potential applications in the food industry. The extractions were carried out in different aqueous organic solvents- ethanol 80% and acetone 80% at optimized conditions. The overall antioxidant capacity of the extracts was estimated by the combined evaluation of Folin-Ciocâlteu total phenol content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing ability of plasma (FRAP) assays using a scoring system. Aqueous ethanol had better extraction efficiency than aqueous acetone as a solvent medium. Of all the investigated species, Quercus robur showed the maximum antioxidant capacity with TPC of 105.88 ± 17.75 mg GAE/g dw, DPPH (IC50) of 1.90 ± 0.10 µg/mL, ABTS of 437.09 ± 36.22 mg TE/g dw and FRAP of 102.62 ± 8.69 mg AAE/g dw. The polyphenolic characterization of Quercus robur, Robinia pseudoacacia and Fraxinus excelsior was done by liquid chromatography/tandem mass spectrometry.



中文翻译:

低频,绿色声纳提取匈牙利林地树皮中的抗氧化剂,可用于潜在食品

本工作评价和比较了匈牙利的十种常见木属(栎属樱桃属血清栎属桦(Betula pendula)优良的Fraxinus)刺槐(Robinia pseudoacacia),桦木(Carpinus betulus)云杉(Picea abies)nu木(Alnus glutinosa),树皮中生物活性成分的抗氧化能力。樟子松)。低频超声用于增强从树皮中的提取,以获得富含多酚类抗氧化剂的提取物,在食品工业中具有潜在的应用。提取是在不同的水性有机溶剂(80%乙醇和80%丙酮)中在最佳条件下进行的。通过对Folin-Ciocâlteu总酚含量(TPC),2,2-二苯基-1-吡啶并肼基(DPPH),2,2'-叠氮基双(3-乙基苯并噻唑啉-使用计分系统进行6-磺酸(ABTS)和血浆铁还原能力(FRAP)分析。与作为溶剂介质的丙酮水溶液相比,乙醇水溶液具有更好的提取效率。在所有调查的物种中,TPC为105.88±17.75 mg GAE / g dw,DPPH(IC 50)为1.90±0.10 µg / mL,ABTS为437.09±36.22 mg TE / g dw,FRAP为102.62±8.69 mg AAE / g时显示最大抗氧化能力dw。用液相色谱/串联质谱法对罗布斯栎,刺槐水曲柳的多酚进行表征。

更新日期:2020-11-09
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