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Progress on the usage of the rotifer Brachionus plicatilis in marine ecotoxicology: A review
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2020-11-07 , DOI: 10.1016/j.aquatox.2020.105678
Xiao-Dong Li , Xin-Yi Wang , Meng-En Xu , Yong Jiang , Tian Yan , Xiao-Cheng Wang

The rotifer, Brachionus plicatilis, is a widely used model species in marine ecotoxicology for evaluating pollutions, toxins, and harmful algae. In this paper, the marine ecotoxicology of Brachionus plicatilis was reviewed, including toxicity measurements of harmful algae species and environmental stresses. In addition, marine pollution involving pesticides, heavy metals, drugs, petroleum, and petrochemicals were addressed. Methods for measuring toxicity were also discussed. The standard acute lethal assay and the chronic population dynamics test were indicated as common methods of toxicity evaluating using B. plicatilis. Research on other biomarkers, such as behaviour, enzyme activity, or gene expression, are also reported here, with potential applications for fast detection or the scientific exploration of underlying molecular mechanisms. It is suggested that the methods selected should reflect the experimental purpose. Additionally, series assays should be conducted for comprehensive evaluation of ecotoxicity as well as to elucidate the correct mechanisms. Genetic methods, such as transcriptomics, were suggested as useful tools for exploring the toxicity mechanism using the rotifer B. plicatilis.



中文翻译:

轮虫Brachionus plicatilis在海洋生态毒理学中的应用进展:综述

轮虫Brachionus plicatilis是海洋生态毒理学中广泛使用的模型物种,用于评估污染,毒素和有害藻类。本文综述了Brachionus plicatilis的海洋生态毒理学,包括有害藻类的毒性测量和环境胁迫。此外,还解决了涉及农药,重金属,药物,石油和石化产品的海洋污染。还讨论了测量毒性的方法。标准的急性致死试验和慢性种群动态试验被指示为使用褶皱双歧杆菌进行毒性评估的常用方法。此处还报告了其他生物标记的研究,例如行为,酶活性或基因表达,具有在快速检测或潜在分子机制的科学探索中的潜在应用。建议所选择的方法应反映实验目的。此外,应进行系列分析以全面评估生态毒性,并阐明正确的机制。遗传方法,如转录组学,被认为是探索使用轮虫B. plicatilis的毒性机制的有用工具。

更新日期:2020-11-15
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