Biofilms are microbial communities that bind to surfaces resist adverse conditions. Increasing the survival of bacteria in biofilm structures compared to their planktonic form causes problems in drug treatment. On the other hand, drug resistance in the world is increasing and the need to discover and identify new compounds with the antimicrobial effect is felt. Marine sponges are adapted to unique marine environments and can fight pathogens of these ecosystems without having a dedicated defense system. This study aimed was to investigate the antimicrobial and anti-biofilm effects and also to identify the bioactive compounds of two samples of Psammocinia sp. and Hyattella sp. sponges. Six bacteria Pseudomonas aeruginosa, Acinetobacter baumanni, Klebsiella pneumonia, Escherichia coli, Staphylococcus aureus and Bacillus cereus were tested. The extract was first extracted using dichloromethane and methanol (DCM: MeOH) (1:1 v/v) solvents. The planktonic form was investigated using Disk diffusion and agar well diffusion methods. The minimum inhibitory concentration (MIC) was determined by the microdilution method and then the minimum bactericidal concentration (MBC). Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS) were performed to identify the compounds of each extract. No zone of inhibition (ZOI) was observed on the planktonic form of K. pneumonia due to both extracts. MIC have about 10 to 20 mg/ml and MBC in about 20 to 80 mg/ml was determined. The results showed that the effect of both extracts on the degradation of the biofilm formed by B. cereus was less than other bacteria. The results of GC-MS showed the presence of phenol, butanedioic acid, propanoic acid and Benzeneacetaldehyde compounds. This study showed that marine sponges at the Persian Gulf can be a good candidate for the extraction of bioactive compounds that use as antimicrobial agents.

生物膜是与表面结合的微生物群落，可抵抗不利条件。与浮游形式相比，增加生物膜结构中细菌的存活率会导致药物治疗方面的问题。另一方面，世界上的抗药性正在增加，并且发现发现和鉴定具有抗微生物作用的新化合物的需求。海洋海绵适用于独特的海洋环境，无需专门的防御系统即可对抗这些生态系统的病原体。这项研究的目的是调查抗菌和抗生物膜的作用，并确定两个沙门氏菌样品的生物活性化合物。和凯悦菌属。海绵。六种铜绿假单胞菌，鲍曼不动杆菌测试了肺炎克雷伯菌，大肠埃希菌，金黄色葡萄球菌和蜡状芽孢杆菌。首先使用二氯甲烷和甲醇（DCM：MeOH）（1：1 v / v）溶剂萃取萃取液。使用圆盘扩散法和琼脂井扩散法研究了浮游形式。通过微量稀释法确定最小抑菌浓度（MIC），然后通过最小杀菌浓度（MBC）确定。进行了气相色谱（GC）和气相色谱-质谱（GC-MS）鉴定每种提取物的化合物。在肺炎克雷伯菌的浮游形式上未观察到抑制区（ZOI）由于两种提取物。MIC约为10至20mg / ml，而MBC约为20至80mg / ml。结果表明，两种提取物对蜡状芽孢杆菌形成的生物膜降解的影响均小于其他细菌。GC-MS的结果表明存在苯酚，丁二酸，丙酸和苯乙醛化合物。这项研究表明，波斯湾的海洋海绵可能是提取用作抗菌剂的生物活性化合物的理想选择。