This study focuses on identifying the factors under which mixed microbial seeds assist bio-chemical denitrification when Scrap Iron Filings (SIF) are used as electron donors and adsorbents in low C/N ratio waters. Batch studies were conducted in abiotic and biotic reactors containing fresh and aged SIF under different dissolved oxygen concentrations with \({\rm{NO}}_3^ - {\rm{- N}}\) and/or PO₄³⁻ influent(s) and their nitrate/phosphate removal and by-product formations were studied. Batch reactors were seeded with a homogenized mixed microbial inoculum procured from natural sludges which were enriched over 6 months under denitrifying conditions in the presence of SIF. Results indicated that when influent containing 40 mg/L of \({\rm{NO}}_3^ - {\rm{- N}}\) was treated with 5 g SIF, 79.9% nitrate reduction was observed in 8 days abiotically and 100% removal was accomplished in 20 days when the reactor was seeded. Both abiotic and seeded reactors removed more than 92% PO₄³⁻ under high DO conditions in 12 days. Abiotic and biochemical removal of \({\rm{NO}}_3^ - {\rm{- N}}\) and abiotic removal of PO₄³⁻ were higher under independent \({\rm{N}}{{\rm{O}}_3}^ - {\rm{- N/P}}{{\rm{O}}_4}^{3 -}\) loading, while 99% PO4³⁻ was removed biochemically under combined \({\rm{N}}{{\rm{O}}_3}^ - {\rm{- N}}\) and PO₄³⁻ loading. This study furthers the understandings of nitrate and phosphate removal in Zero Valent Iron (ZVI) assisted mixed microbial systems to encourage the application of SIF-supported bio-chemical processes in the simultaneous removals of these pollutants.

这项研究的重点是确定在低C / N比水中将废铁屑（SIF）用作电子供体和吸附剂时，混合微生物种子协助生化反硝化的因素。在装有新鲜和老化的SIF的非生物和生物反应堆中，使用\（{\ rm {NO}} _ 3 ^-{\ rm {-N}} \）和/或PO ₄ ^3-进水，在不同的溶解氧浓度下进行分批研究研究了它们的硝酸盐/磷酸盐去除和副产物形成。分批反应器用从天然污泥获得的均质混合微生物接种物接种，该接种物在SIF存在下于反硝化条件下在6个月内富集。结果表明，当进水中含有40 mg / L的\（{\ rm {NO}} _ 3 ^-{\ rm {-N}} \）用5g SIF处理有机硅，非生物性地在8天内观察到硝酸盐减少了79.9％，并且在接种反应器时在20天内完成了100％的去除。在高溶解氧条件下，非生物和种子反应堆在12天内都去除了92％以上的PO ₄ ^3-。\（{\ rm {NO}} _ 3 ^-{\ rm {-N}} \）的非生物和生化去除和PO ₄ ^3−的非生物去除在独立的\（{\ rm {N}} {{ \ rm {O}} _ 3} ^-{\ rm {-N / P}} {{\ rm {O}} _ 4} ^ {3-} \）负载，而99％PO4 ^3-在生化条件下通过组合化学去除\（{\ rm {N}} {{\ rm {O}} _ 3} ^-{\ rm {-N}} \）和PO ₄ ³⁻加载中。这项研究进一步了解了零价铁（ZVI）辅助混合微生物系统中硝酸盐和磷酸盐的去除方法，以鼓励SIF支持的生物化学过程在同时去除这些污染物中的应用。