There are concerns regarding the toxicity of household detergents to humans and aquatic organisms. There are various types of surfactants, which are the main components of detergents, and each detergent product has a different formulation. Therefore, high-throughput technologies capable of analyzing a large number of samples rapidly and accurately are required for research on detergent toxicity. In this study, image analysis techniques were used to quantify the locomotor activities from captured images using larval zebrafish (Danio rerio). When the 135-min time-lapse imaging was completed, the damage to the body of the animals was examined using a microscope. Additionally, we determined the animal survival and LC₅₀. Nonionic decyl glucoside (APG-C10), one of the alkyl polyglucosides (APGs), exhibited the lowest toxicity, whereas linear alcohol ethoxylate (LA-9) exhibited the highest toxicity. AOS (α-olefin sulfonate), LAS (linear alkylbenzene sulfonate), and APG-600 showed moderate toxicity between LA-9 and APG-C10. Commercial detergents exhibited various degrees of toxicity, and there was a linear relationship (R² = 0.776) between locomotor activity and survival percentage of animals.

人们担心家用洗涤剂对人类和水生生物的毒性。表面活性剂有多种类型，它们是洗涤剂的主要成分，每种洗涤剂产品的配方都不同。因此，对于洗涤剂毒性的研究需要能够快速，准确地分析大量样品的高通量技术。在这项研究中，图像分析技术被用来量化使用幼虫斑马鱼（Danio rerio）从捕获的图像中的运动活动。完成135分钟的延时成像后，使用显微镜检查对动物身体的伤害。此外，我们确定了动物的存活率和LC ₅₀。烷基多​​葡糖苷（APG）之一的非离子癸基葡糖苷（APG-C10）毒性最低，而直链醇乙氧基化物（LA-9）毒性最高。AOS（α-烯烃磺酸盐），LAS（线性烷基苯磺酸盐）和APG-600在LA-9和APG-C10之间显示中等毒性。商业洗涤剂表现出不同程度的毒性，运动活性与动物存活率之间存在线性关系（R ² = 0.776）。