The use of microbial phosphotriesterases in the degradation of organophosphorus compounds employed as pesticides, plasticizers and petroleum additives is a sustainable alternative for bioremediation of water and soils, decontamination of particular foods and as poisoning antidote. Whole cells of six wild type microorganisms—Streptomyces phaeochromogenes, Streptomyces setonii, Nocardia corynebacterioides, Nocardia asteroides and two Arthrobacter oxydans—selected in our lab as phosphotriesterase sources, were further tested as biocatalysts in the hydrolysis of paraoxon, methyl paraoxon, methyl parathion, coroxon, coumaphos, dichlorvos and chlorpyrifos, highlighting 98% conversion of chlorpyrifos into its hydrolysis products using whole cells of S. phaeochromogenes at pH 8 and 40 °C. Immobilized whole cells and enzyme extracts were also assessed, observing as a general trend, that there is no significant variation in hydrolytic activity between them. These results suggest that according to the circumstances, immobilized whole cells (avoiding cellular disruption and centrifugation) or enzyme extracts (which can be handled more easily) could be used.

在降解用作农药，增塑剂和石油添加剂的有机磷化合物时，使用微生物磷酸三酯酶是对水和土壤进行生物修复，对特定食品进行净化和用作有毒解毒剂的可持续选择。六野生型microorganisms-整个电池链phaeochromogenes，链霉菌setonii，诺卡corynebacterioides，星状诺卡氏菌和两个氧化节杆菌-在我们的实验室中被选作磷酸三酯酶的来源，在对氧磷，甲基对氧磷，甲基对硫磷，反氧磷，香豆磷，敌敌畏和毒死rif的水解中作为生物催化剂进行了进一步测试，强调了利用S的整个细胞将毒死rif 98％转化为其水解产物。 phaeochromogenes在pH 8和40℃。还观察到固定化的全细胞和酶提取物，总体趋势是，它们之间的水解活性没有明显变化。这些结果表明，根据情况，可以使用固定的全细胞（避免细胞分裂和离心分离）或酶提取物（可以更轻松地处理）。