American Journal of Potato Research ( IF 1.2 ) Pub Date : 2020-11-09 , DOI: 10.1007/s12230-020-09808-x Norma Manrique-Carpintero , Md Shafiqul Islam , Felix Enciso-Rodríguez , Noah Rosenzweig , Joseph Coombs , David S. Douches
Late blight, caused by the oomycete Phytophthora infestans (Mont) de Bary, is the most important disease of cultivated potato (Solanum tuberosum L.). An efficient long-term strategy for controlling late blight infection involves integration of host plant resistance to disease management programs. In this study, a diploid potato population segregating for late blight resistance was generated from an interspecific cross (MSX902) between S. tuberosum x S. chacoense hybrid (84SD22) and a late blight resistant Solanum berthaultii hybrid (Ber83). A total of 129 progeny and two parents were tested for foliar resistance against the US-23 P. infestans genotype using detached-leaf bioassays and inoculated field trials (2014 and 2015). Distribution of foliar late blight resistance and susceptibility was bimodal in both types of phenotype evaluation, suggesting a major R-gene may be associated with the resistance. A major QTL for late blight resistance on chromosome 10 was detected using both detached leaf bioassay and field trial data. The SNP marker solcap_snp_c1_15219 (26.5 cM) was at the peak of the QTL. Additionally, a minor QTL on chromosomes 5 was linked to SNPs solcap_snp_c1_3793. These SNP markers closely linked to the late blight resistance QTL can be used for marker-assisted breeding.
中文翻译:
在二倍体种群中绘制基于berthaultii的茄属晚疫病(疫疫霉)抗性
由卵菌疫霉疫霉(Monty de Bary)引起的晚疫病是栽培马铃薯(Solanum tuberosum L.)的最重要疾病。控制晚疫病的有效长期策略涉及宿主植物对疾病管理计划的抵抗力的整合。在该研究中,从之间的种间杂交(MSX902)产生的二倍体马铃薯人口偏析晚疫病抗性马铃薯X S. chacoense杂交体(84SD22)和抗晚疫病茄berthaultii杂交体(Ber83)。对总共129个后代和两个亲本进行了针对US-23 P. infestans的叶面抗药性测试分离叶生物测定和接种田间试验的基因型(2014年和2015年)。在两种表型评估中,叶晚疫病抗性和敏感性的分布均为双峰分布,表明主要的R基因可能与抗性有关。使用离体叶片生物测定法和田间试验数据检测了10号染色体上晚疫病抗性的主要QTL。SNP标记solcap_snp_c1_15219(26.5 cM)位于QTL的峰值。此外,第5号染色体上的次要QTL与SNP solcap_snp_c1_3793相关。这些与晚疫病抗性QTL紧密相关的SNP标记可用于标记辅助育种。