Purpose

The aim of this study was to isolate Babesia canis soluble antigens and to investigate the effect of their conjugates with gold nanoparticles on the immunogenicity in laboratory animals.

Methods

A procedure was developed for isolating and purifying B. canis antigens. The isolated culture antigen of B. canis 495 was coupled to gold nanoparticles, and the conjugate was used to immunize laboratory mice.

Results

Western blotting showed that the resultant antiserum specifically recognized the proteins of the B. canis strains isolated from naturally infected dogs. The antibody titer, the respiratory activity of peritoneal macrophages, the proliferative activity of splenocytes, and the production of cytokines were maximal when the animals were immunized with the antigen–nanoparticle conjugate emulsified in complete Freund’s adjuvant. Without adjuvant, the babesial antigen was weakly immunogenic.

Conclusion

Therefore, the use of gold nanoparticles as an antigen carrier induced a broad immune response involving both cellular and humoral responses. The antibodies raised by the proposed procedure are potentially effective at immunodetection of Babesia canis infections in dogs.

中文翻译：

---

当抗原与金纳米颗粒偶联时，小鼠对犬巴贝斯虫抗原的免疫反应增强

目的

本研究的目的是分离犬巴贝斯虫可溶性抗原，并研究它们与金纳米颗粒的偶联物对实验室动物免疫原性的影响。

方法

开发了一种用于分离和纯化犬双歧杆菌抗原的程序。B. canis 495的分离培养抗原与金纳米粒子偶联，该偶联物用于免疫实验室小鼠。

结果

蛋白质印迹表明，产生的抗血清特异性识别从自然感染的狗身上分离的犬双歧杆菌菌株的蛋白质。当用完全弗氏佐剂乳化的抗原-纳米颗粒偶联物免疫动物时，抗体滴度、腹膜巨噬细胞的呼吸活性、脾细胞的增殖活性和细胞因子的产生最大。在没有佐剂的情况下，巴贝斯虫抗原是弱免疫原性的。

结论

因此，使用金纳米粒子作为抗原载体可诱导广泛的免疫反应，包括细胞和体液反应。由提议的程序产生的抗体在犬巴贝斯虫感染的免疫检测方面可能有效。