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Effect of rest temperature on rotational thromboelastometry in New Zealand White rabbits
The Journal of Veterinary Diagnostic Investigation ( IF 1.2 ) Pub Date : 2020-11-06 , DOI: 10.1177/1040638720968536
Kelsea A Studer 1 , Andrew Hanzlicek 1 , Nicola Di Girolamo 1 , William Womble 1 , Debosree Pathak 1 , Rachel Maranville 1 , Ian Kanda 1 , João Brandão 1
Affiliation  

With the increasing popularity of viscoelastic coagulation analyzers, such as rotational thromboelastometry [ROTEM] and thromboelastography, the need for standardized methodology for appropriate interpretation has become increasingly important. Viscoelastic analysis is heavily influenced by a multitude of pre-analytic factors, both in vivo and in vitro, leading to a large amount of variation between institutions. We investigated the effect of room temperature during a 30-min sample rest time on ROTEM, which analyzed both intrinsic and extrinsic pathways. We also evaluated the feasibility of using ROTEM to assess coagulation in non-anesthetized domestic rabbits. Rabbits were selected because they are a common companion animal that could benefit from the use of viscoelastic analysis for various disease processes that could lead to coagulopathies. Citrated whole blood was collected from 10 rabbits and allowed to rest upright for 30 min either at room temperature (~ 21°C) or in a tube warmer (37°C) before analysis. There was no significant difference in results between room temperature and warmed samples, which suggests that allowing samples to rest at room temperature is acceptable clinically. Additionally, blood collection and analysis were feasible in all rabbits.



中文翻译:

静止温度对新西兰白兔旋转血栓弹力图的影响

随着粘弹性凝血分析仪(如旋转血栓弹力图 [ROTEM] 和血栓弹力图)的日益普及,对适当解释的标准化方法的需求变得越来越重要。粘弹性分析受到许多体内和体外分析前因素的严重影响,导致机构之间存在大量差异。我们研究了室温在 30 分钟样品静止时间内对 ROTEM 的影响,ROTEM 分析了内在和外在途径。我们还评估了使用 ROTEM 评估非麻醉家兔凝血的可行性。选择兔子是因为它们是一种常见的伴侣动物,可以从对可能导致凝血病的各种疾病过程进行粘弹性分析中受益。从 10 只兔子收集柠檬酸化全血,并在分析前在室温 (~ 21°C) 或管式加热器 (37°C) 中直立静置 30 分钟。室温和加热样品之间的结果没有显着差异,这表明让样品在室温下静置在临床上是可以接受的。此外,所有兔子的血液收集和分析都是可行的。

更新日期:2020-11-06
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