Objective. Our study was aimed at investigating the mechanistic consequences of the upregulation of adipocyte enhancer-binding protein 1 (AEBP1) in glioblastoma (GBM). Methods. The expression of AEBP1 in GBM was assessed by bioinformatics analysis and qRT-PCR; the effects of AEBP1 on GBM cell proliferation, migration, invasion, and tumor growth in vitro and in vivo were detected by a CCK-8 assay, colony formation assay, scratch assay, Transwell assay, and subcutaneous tumor formation, respectively. The activation of related signaling pathways was monitored using western blot. Results. Tumor-related databases and bioinformatics analysis revealed that AEBP1 was highly expressed in GBM and indicated poor outcome of patients; its high expression that was also confirmed in GBM tissues and cell lines was closely related to the tumor size. The results of in vitro experiments showed that AEBP1 could significantly promote GBM cell proliferation, migration, and invasion; in vivo experiments suggested that AEBP1 could contribute to the growth of GBM tumors. AEBP1 could upregulate the level of IκBα phosphorylation, decrease IκBα expression, activate the NF-κB signaling pathway, and promote the expression of downstream oncogenes. Conclusion. Upregulated AEBP1 in GBM promotes GBM cell proliferation, migration, and invasion and facilitates tumor growth in vivo by activating the classical NF-κB pathway.

中文翻译：

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AEBP1 促进胶质母细胞瘤进展并激活经典 NF-κB 通路

客观。我们的研究旨在调查胶质母细胞瘤 (GBM)中脂肪细胞增强子结合蛋白 1 ( AEBP1 ) 上调的机制后果。方法。通过生物信息学分析和qRT-PCR评估GBM中AEBP1的表达；AEBP1对体外和体内GBM细胞增殖、迁移、侵袭和肿瘤生长的影响分别通过CCK-8试验、集落形成试验、划痕试验、Transwell试验和皮下肿瘤形成进行检测。使用蛋白质印迹监测相关信号通路的激活。结果。肿瘤相关数据库和生物信息学分析表明，AEBP1在 GBM 中高度表达，表明患者预后不良；其在GBM组织和细胞系中的高表达也与肿瘤大小密切相关。体外实验结果表明，AEBP1可显着促进GBM细胞增殖、迁移和侵袭；体内实验表明，AEBP1可能有助于 GBM 肿瘤的生长。AEBP1可上调IκBα磷酸化水平，降低IκBα表达，激活NF-κB信号通路，促进下游癌基因表达。结论。上调AEBP1在 GBM 中，通过激活经典的 NF- κB通路促进 GBM 细胞增殖、迁移和侵袭，并促进体内肿瘤生长。