Abstract Introduction To date, there have been no panoramic studies of the serum metabolome in feline mammary carcinoma. As the first such study, metabolomics techniques were used to analyse the serum of cats with these tumours. Three important metabolic pathways of screened differential metabolites closely related to feline mammary carcinomas were analysed to lay a theoretical basis for further study of the pathogenesis of these carcinomas. Material and Methods Blood in a 5–8 mL volume was sampled from twelve cats of the same breed and similar age (close to nine years on average). Six were feline mammary carcinoma patients and six were healthy. L glutamate, L alanine, succinate, adenine, hypoxanthine, and inosine were screened as were alanine, aspartate, and glutamate metabolism, the tricarboxylid acid (TCA) cycle, and purine metabolism. Data were acquired with LC-MS non-target metabolomics, multiple reaction monitoring target metabolomics, and multivariate statistical and bioinformatic analysis. Results Expression of five of the metabolites was upregulated and only inosine expression was downregulated. Up- and downregulation of metabolites related to glycometabolism, potentiation of the TCA cycle, greater content of lipid mobilisation metabolites, and abnormality of amino acid metabolism were closely related to the occurrence of the carcinomas. Conclusion These findings provide a new direction for further study of the mechanisms associated with cat mammary neoplasms.

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基于 LC-MS 和 MRM 技术的猫乳腺癌血清代谢组学分析

摘要 引言 迄今为止，尚无关于猫乳腺癌血清代谢组的全景研究。作为第一项此类研究，代谢组学技术被用于分析患有这些肿瘤的猫的血清。分析筛选出的与猫乳腺癌密切相关的差异代谢物的三个重要代谢途径，为进一步研究这些癌的发病机制奠定理论基础。材料和方法 从 12 只相同品种和相似年龄（平均接近 9 岁）的猫中抽取 5-8 mL 体积的血液。六名是猫乳腺癌患者，六名是健康的。L 谷氨酸、L 丙氨酸、琥珀酸盐、腺嘌呤、次黄嘌呤和肌苷以及丙氨酸、天冬氨酸和谷氨酸代谢、三羧酸 (TCA) 循环和嘌呤代谢进行了筛选。通过 LC-MS 非目标代谢组学、多反应监测目标代谢组学以及多变量统计和生物信息学分析获取数据。结果五种代谢物的表达上调，只有肌苷的表达下调。与糖代谢相关的代谢物的上调和下调、TCA循环的增强、脂质动员代谢物含量的增加以及氨基酸代谢的异常与癌的发生密切相关。结论 这些发现为进一步研究猫乳腺肿瘤的相关机制提供了新的方向。结果五种代谢物的表达上调，只有肌苷的表达下调。与糖代谢相关的代谢物的上调和下调、TCA循环的增强、脂质动员代谢物含量的增加以及氨基酸代谢的异常与癌的发生密切相关。结论 这些发现为进一步研究猫乳腺肿瘤的相关机制提供了新的方向。结果五种代谢物的表达上调，只有肌苷的表达下调。与糖代谢相关的代谢物的上调和下调、TCA循环的增强、脂质动员代谢物含量的增加以及氨基酸代谢的异常与癌的发生密切相关。结论 这些发现为进一步研究猫乳腺肿瘤的相关机制提供了新的方向。