Abstract

Aims

To prepare a novel antimicrobial peptide Nal-P-113 loaded poly (ethylene glycol) combined chitosan nanoparticles (Nal-P-113-PEG-CSNPs) for root caries restorations to control the periodontitis related pathogens in periodontitis care.

Methods

Nanoparticles were prepared by simple polymerisation method and characterised using effective analytical methods (TEM, UV, etc.). The antimicrobial activity and biofilm formation of Nal-P-113-PEG-CSNPs was tested against periodontal bacterial pathogens by different in vitro methods.

Results

The size of Nal-P-113 loaded PEG-Chitosn nanoparticles was 216.2 ± 1.6 nm. The drug encapsulation efficiency (%EE (w/w) of Nal-P-113-PEG-CSNPs was found to be 89.33 ± 1.67% (w/w). The antimicrobial examination showed that prepared NPs have effectively inhibited the growth of Fusobacterium nucleatum, Streptococcus gordonii, and Porphyromonas gingivalis with the MIC of 23 µg/mL, 6 µg/mL and 31 µg/mL, respectively.

Conclusions

The prepared antimicrobial peptide-loaded PEG-CSNPs provide excellent in vitro efficiency but, further studies are necessary to confirm its therapeutic efficacy on periodontitis care.

中文翻译：

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牙周病护理中用于根面龋修复的抗菌肽纳米材料抑制牙周炎相关病原体的研究

摘要

宗旨

制备一种新型抗菌肽负载Nal-P-113的聚（乙二醇）复合壳聚糖纳米颗粒（Nal-P-113-PEG-CSNPs）用于根龋修复，以控制牙周炎护理中的牙周炎相关病原体。

方法

纳米颗粒通过简单的聚合方法制备，并使用有效的分析方法（TEM、UV 等）进行表征。通过不同的体外方法测试了 Nal-P-113-PEG-CSNPs 对牙周细菌病原体的抗菌活性和生物膜形成。

结果

负载 Nal-P-113 的 PEG-壳聚糖纳米颗粒的尺寸为 216.2 ± 1.6 nm。发现Nal-P-113-PEG-CSNPs的药物包封率（%EE（w/w）为89.33±1.67%（w/w）。抗菌检测表明制备的NPs有效抑制梭杆菌的生长nucleatum、Streptococcus gordonii和Porphyromonas gingivalis的 MIC 分别为 23 µg/mL、6 µg/mL 和 31 µg/mL。

结论

制备的载有抗菌肽的 PEG-CSNPs 具有出色的体外效率，但需要进一步研究以证实其对牙周炎护理的治疗效果。