Abstract Terpenes produced by plants comprise a diverse range of secondary metabolites, including volatile organic compounds (VOCs). Terpene VOC production may be altered after damage or by biological stimuli such as bacterial, fungal and insects, and subsequent triggering of plant defense responses. These VOCs originate in plants from two independent pathways: the mevalonate and the methylerythritol phosphate pathways, which utilize dimethylallyl and isopentenyl diphosphates to form the terpenoidal precursors. Phakopsora pachyrhizi fungi causes Asian soybean rust, limiting soybean production and resulting in losses of up to 80% if no control strategies are applied. By using a transcriptome datasets, we investigated the regulation of genes of the mevalonate pathway under different biotic stresses. We studied the impact of P. pachyrhizi infection in vivo expression profile of genes involved in terpenoid and glyceollin biosynthesis in genotypes harboring different resistance genes (Rpp), and across the infection cycle. In addition, we used UPLC and UPGC analysis to evaluate glyceollin and VOC production, respectively, to identify metabolites associated with soybean responses to pathogen infection. The regulation of soybean genes involved in terpene production was influenced by genotypes, depending on the Rpp gene, while glyceollin was induced in all genotypes. Furthermore, a sesquiterpene was identified as a potential marker associated with rust symptoms on soybean.

中文翻译：

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大豆生物胁迫下参与萜烯和甘油生产的基因转录谱

摘要 植物产生的萜烯包括多种次级代谢产物，包括挥发性有机化合物 (VOC)。萜烯 VOC 的产生可能会在受到损害或受到细菌、真菌和昆虫等生物刺激以及随后触发植物防御反应的影响后发生改变。这些 VOC 源自植物中的两个独立途径：甲羟戊酸和甲基赤藓糖醇磷酸途径，它们利用二甲基烯丙基和异戊烯基二磷酸形成萜类前体。Phakopsora pachyrhizi 真菌导致亚洲大豆锈病，限制大豆产量，如果不采取控制策略，将导致高达 80% 的损失。通过使用转录组数据集，我们研究了不同生物胁迫下甲羟戊酸途径基因的调控。我们研究了 P 的影响。pachyrhizi 感染体内参与萜类和甘油生物合成的基因在具有不同抗性基因 (Rpp) 的基因型中的表达谱，以及整个感染周期。此外，我们使用 UPLC 和 UPGC 分析分别评估甘油和 VOC 的产生，以确定与大豆对病原体感染的反应相关的代谢物。参与萜烯生产的大豆基因的调控受基因型的影响，取决于 Rpp 基因，而甘油三酯在所有基因型中都被诱导。此外，倍半萜烯被确定为与大豆锈病症状相关的潜在标志物。我们使用 UPLC 和 UPGC 分析分别评估甘油和 VOC 的产生，以确定与大豆对病原体感染的反应相关的代谢物。参与萜烯生产的大豆基因的调控受基因型的影响，取决于 Rpp 基因，而甘油三酯在所有基因型中都被诱导。此外，倍半萜烯被确定为与大豆锈病症状相关的潜在标志物。我们使用 UPLC 和 UPGC 分析分别评估甘油和 VOC 的产生，以确定与大豆对病原体感染的反应相关的代谢物。参与萜烯生产的大豆基因的调控受基因型的影响，取决于 Rpp 基因，而甘油三酯在所有基因型中都被诱导。此外，倍半萜烯被确定为与大豆锈病症状相关的潜在标志物。