SNP‐based genotyping and whole‐genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin,Plant Pathology

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SNP‐based genotyping and whole‐genome sequencing reveal previously unknown genetic diversity in Xanthomonas vasicola pv. musacearum, causal agent of banana xanthomonas wilt, in its presumed Ethiopian origin
Plant Pathology ( IF 2.3 ) Pub Date : 2020-11-05 , DOI: 10.1111/ppa.13308
Gloria V Nakato _{1,

2} , David J Studholme ₃ , Guy Blomme ₄ , Murray Grant ₅ , Teresa A Coutinho ₂ , Evans M Were ₁ , Emmanuel Wicker _{6,

7} , George Mahuku _{1,

8}

Affiliation

For decades, Xanthomonas vasicola pv. musacearum (Xvm) has been an economically important bacterial pathogen on enset in Ethiopia. Since 2001, Xvm has also been responsible for significant losses to banana crops in several East and Central African countries, with devastating consequences for smallholder farmers. Understanding the genetic diversity within Xvm populations is essential for the smart design of transnationally reasoned, durable, and effective management practices. Previous studies have revealed limited genetic diversity in Xvm, with East African isolates from banana each falling into one of two closely related clades previously designated as sublineages SL 1 and SL 2, the former of which had also been detected on banana and enset in Ethiopia. Given the presumed origin of Xvm in Ethiopia, we hypothesized that both clades might be found in that country, along with additional genotypes not seen in Central and East African bananas. Genotyping of 97 isolates and whole‐genome sequencing of 15 isolates revealed not only the presence of SL 2 in Ethiopia, but additional diversity beyond SL 1 and SL 2 in four new clades. Moreover, SL 2 was detected in the Democratic Republic of Congo, where previously SL 1 was the only clade reported. These results demonstrate a greater range of genetic diversity among Xvm isolates than previously reported, especially in Ethiopia, and further support the hypothesis that the East/Central Africa xanthomonas wilt epidemic has been caused by a restricted set of genotypes drawn from a highly diverse pathogen pool in Ethiopia.

中文翻译：

基于 SNP 的基因分型和全基因组测序揭示了黄单胞菌 pv 中以前未知的遗传多样性。 musacearum，香蕉黄单胞菌枯萎病的病原体，推测起源于埃塞俄比亚

几十年来，黄单胞菌pv。 musacearum (Xvm) 是埃塞俄比亚流行的一种重要的经济细菌病原体。自 2001 年以来，Xvm 还对一些东非和中非国家的香蕉作物造成重大损失，给小农带来了毁灭性后果。了解 Xvm 种群内的遗传多样性对于智能设计跨国合理、持久和有效的管理实践至关重要。先前的研究表明 Xvm 的遗传多样性有限，东非香蕉分离株均属于先前指定为 SL 1 和 SL 2 亚系的两个密切相关的分支之一，前者也在埃塞俄比亚的香蕉和 enset 中检测到。鉴于 Xvm 假定起源于埃塞俄比亚，我们假设这两个进化枝都可能在该国发现，以及中非和东非香蕉中未发现的其他基因型。对 97 个分离株的基因分型和 15 个分离株的全基因组测序不仅揭示了埃塞俄比亚存在 SL 2，而且在四个新进化枝中还存在 SL 1 和 SL 2 之外的额外多样性。此外，SL 2 在刚果民主共和国被发现，而此前 SL 1 是该国报告的唯一进化枝。这些结果表明，Xvm 分离株之间的遗传多样性比之前报道的更大，特别是在埃塞俄比亚，并进一步支持了这样的假设：东非/中非黄单胞菌枯萎病流行是由来自高度多样化的病原体库的一组有限基因型引起的。在埃塞俄比亚。

更新日期：2020-11-05

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