In the present study, an integrated process for preparation of anthocyanins components from blue honeysuckle fruits using deep eutectic solvent-based negative pressure cavitation extraction (DES-NPCE) was explored rationally. Single factor optimization combined with Box-Behnken Design (BBD) were applied to confirm the influential parameters. The maximum extraction yields of total anthocyanins and main constituent cyanidin-3-glucoside (C3G) reached 6.601 mg/g and 4.839 mg/g, which were 1.197–1.565 folds of other conventional methods. Moreover, the anthocyanins products exhibited higher stability from DES-NPCE extraction than that of conventional extraction solvents, which was conducive to the preservation and utilization of anthocyanins. After enrichment with macroporous resin D101, the contents of total anthocyanins and C3G reached 25.72% and 19.05%, and the recovery yields were 81.35% and 82.24%, respectively. Furthermore, the anthocyanins products exhibited more powerful chemical and cellular antioxidant activities, which were similar or stronger than that of positive control Vc or BHT. These results revealed that the proposed process was definitely an efficient and sustainable strategy for the stabilization preparation of thermo sensitive biologically active natural products. The developed method has also proved to have sufficient potential for application in food, nutrition and pharmaceutical industries.

在本研究中，合理地探索了一种基于深共熔溶剂的负压空化萃取（DES-NPCE）从蓝色金银花果实中制备花色苷成分的综合方法。应用单因素优化与Box-Behnken设计（BBD）相结合来确定影响参数。总花色苷和主要成分花青素-3-葡萄糖苷（C3G）的最大提取量分别达到6.601 mg / g和4.839 mg / g，是其他常规方法的1.197-1.565倍。此外，花色苷产品在DES-NPCE萃取中表现出比常规萃取溶剂更高的稳定性，这有利于花色苷的保存和利用。用大孔树脂D101富集后，总花色苷和C3G的含量分别达到25.72％和19。05％，回收率分别为81.35％和82.24％。此外，花色苷产品表现出更强的化学和细胞抗氧化活性，与阳性对照Vc或BHT相似或更强。这些结果表明，所提出的方法绝对是稳定制备热敏性生物活性天然产物的有效且可持续的策略。事实证明，所开发的方法在食品，营养和制药行业中具有足够的应用潜力。这些结果表明，所提出的方法绝对是稳定制备热敏性生物活性天然产物的有效且可持续的策略。事实证明，所开发的方法在食品，营养和制药行业中具有足够的应用潜力。这些结果表明，所提出的方法绝对是稳定制备热敏性生物活性天然产物的有效且可持续的策略。事实证明，所开发的方法在食品，营养和制药行业中具有足够的应用潜力。