Seed coat impermeability (SCI) in soybean is associated with seed viability under storage and quality of processed products. Understanding genetics and identification of linked molecular markers would facilitate need-based utilization of seed coat impermeability. Two impermeable wild type (G. soja Sieb. and Zucc.) accessions viz. PI 424079 and PI 136620 were crossed with a permeable cultivated (G. max) variety JS335 to generate the mapping populations. Genetic analysis of the F_1:2 and F_2:3 seeds of the crosses indicated that SCI is controlled by a single gene/major QTL, and impermeability is dominant over permeability. Presence of seeds with intermediate permeability indicated role of some minor genes/QTLs. A set of 204 inter-specific recombinant inbred line (RILs) (F₇) was used to map SCI with 207 SSR markers. Phenotyping through rapid imbibition approach (seed imbibition for 6 h), seven QTLs were mapped on chromosomes (Chrs.) 2, 5, 12, 13 and 16 in the seeds stored for 1–3 years, while through slow imbibition method (seed imbibition for 7 days), five QTLs were mapped on Chrs. 2, 9, 10 and 20. Phenotypic variation explained (PVE) by the QTLs ranged from 5.96 to 39.67%. Two major and stable QTLs viz., qScI-h2-1 and qScI-h2-2 that mapped in tandem on Chr.2 jointly explained 43.09–62.92% of the variations in impermeability. Seven minor QTLs identified here were novel and two (qScI-h5, and qScI-h16) were consistent. It is the first report of mapping impermeability using two imbibition approaches together in 200 plus inter-specific RILs in soybean. The study will pave the way for developing genotypes with restricted permeability, enhanced seed viability, and improved seeds quality.

大豆的种皮抗渗性 (SCI) 与储存条件下的种子活力和加工产品的质量有关。了解遗传学和连锁分子标记的鉴定将促进种皮抗渗性的基于需求的利用。两个不可渗透的野生型（G. soja Sieb. 和 Zucc.）。即。PI 424079 和 PI 136620 与可渗透栽培 ( G. max ) 品种 JS335 杂交以生成作图种群。F _1:2和 F _{2:3 的}遗传分析杂交种子表明 SCI 受单个基因/主要 QTL 控制，并且不渗透性优于渗透性。具有中等渗透性的种子的存在表明一些次要基因/QTL 的作用。一组 204 个特异重组自交系 (RIL) (F ₇) 用于用 207 个 SSR 标记绘制 SCI。通过快速吸收方法（种子吸收 6 h）进行表型分析，在储存 1-3 年的种子中，7 个 QTL 被定位在染色体（Chrs.）2、5、12、13 和 16 上，而通过慢速吸收方法（种子吸收） 7 天），5 个 QTL 被定位在 Chrs 上。2、9、10 和 20。由 QTL 解释的表型变异 (PVE) 范围从 5.96% 到 39.67%。两个主要且稳定的 QTL，即在 Chr.2 上串联映射的 qScI-h2-1 和 qScI-h2-2，共同解释了 43.09-62.92% 的不渗透性变化。这里确定的七个小 QTL 是新的，两个（qScI-h5 和 qScI-h16）是一致的。这是第一份使用两种吸水方法在大豆中 200 多个种间 RIL 中绘制不渗透性的报告。