We build a novel fluorescence resonance energy transfer (FRET) method based on N-doped graphene quantum dots (NGQDs)–MnO₂ nanocomposite for rapid, sensitive detection of glutathione (G SH) levels in human serum. In this strategy, MnO₂ nanosheets on the NGQDs surface serve as a quencher. NGQDs fluorescence can make a recovery by the addition of GSH, which can reduce MnO₂ to Mn²⁺, and thus the GSH can be monitored. The MnO₂ platform affords minimal background and high sensitivity for detecting GSH in this proposed scheme. Meanwhile, relevant fluorescence on–off–on processes were monitored, and the sensing mechanism was explored.

我们建立了一种基于N掺杂石墨烯量子点（NGQDs）-MnO ₂纳米复合材料的新型荧光共振能量转移（FRET）方法，用于快速，灵敏地检测人血清中的谷胱甘肽（G SH）水平。在这种策略中，NGQDs表面的MnO ₂纳米片可用作淬灭剂。NGQDs的荧光可以通过添加GSH来恢复，GSH可以将MnO ₂还原为Mn ²⁺，因此可以监测GSH。MnO ₂平台在此拟议方案中为检测GSH提供了最小的背景和高灵敏度。同时，对相关的荧光开关过程进行了监测，并探讨了其传感机制。