Abstract

While various polyesters have been electrospun into fibrous scaffolds, these constructs lack sufficient strength and osteoconductivity that is usually desired for potential bone tissue engineering. Here, hydroxyapatite (HA), either in the form of traditional nanospheres or in the form of nanorods, was tried to incorporate into a new nanofibrous scaffold based on electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with a given 3-hydroxyvalerate (HV) content. The rod-like nanoparticles were used, in particular, to closely mimic the natural structure of bone. Since the solubility of polymers is a critical parameter in the electrospinning process, the solubility of PHBV having different HV contents was also examined in different solvents using a combined experimental and theoretical study. According to the results, 1,1,1,3,3,3-hexafluoropropan-2-ol is ideally able to dissolve PHBV with 5% HV content and hence to produce nanofibers with minimal structural defects during the electrospinning process. Moreover, although both the spherical and rod-like particles could be homogeneously embedded in the PHBV at the expected concentration, the addition of rod-like HA to PHBV can result in composite nanofibers with a significantly enhanced structural strength.

Graphic Abstract

中文翻译：

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具有增强的结构稳定性的调制复合纳米纤维，可促进硬组织愈合

摘要

尽管各种聚酯已经被静电纺制成纤维状支架，但是这些构造物缺乏足够的强度和骨传导性，这通常是潜在的骨组织工程所需要的。在这里，尝试以传统纳米球形式或纳米棒形式的羟基磷灰石（HA）掺入基于电纺聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）的新型纳米纤维支架中。给定3-羟基戊酸酯（HV）含量。棒状纳米颗粒尤其用于紧密模拟骨骼的天然结构。由于聚合物的溶解度是静电纺丝过程中的关键参数，因此，结合实验和理论研究，还对具有不同HV含量的PHBV在不同溶剂中的溶解度进行了研究。根据结果​​1、1、1、3、3 3-六氟丙烷-2-醇理想地能够溶解HV含量为5％的PHBV，因此可在电纺丝过程中生产出结构缺陷最少的纳米纤维。而且，尽管球形和棒状颗粒都可以以期望的浓度均匀地嵌入PHBV中，但是向PHBV中添加棒状HA可以产生具有显着增强的结构强度的复合纳米纤维。

图形摘要