Abstract

Bacterial cellulose (BC) is a biodegradable polymer that benefits in purity, crystallinity and superior optical, structural and mechanical properties. Such properties facilitate BC to replace the conventional non-biodegradable materials used, for instance, in sensing applications. However, BC production is largely conducted in conventional medium containing model substrates and complex carbon-containing compounds. Aiming towards the production of eco-friendly piezoelectric-responsive BC films, we isolated and characterized a novel bacterial strain affiliated to Komagataeibacter rhaeticus. The K. rhaeticus ENS9a strain synthesized BC in minimal medium containing crude glycerol, generating a titer of 2.9 ± 0.3 g/L BC. This is, to the best of our knowledge, the highest BC titer reported from an unoptimized minimal medium containing crude glycerol. Interestingly, the films prepared from crude glycerol showed normal force and bending mode sensitivities of 6–11 pC/N and 40–71 pC/N, respectively, demonstrating a green platform to address both bioprocess waste valorization and implementation of cellulose-based alternatives for the non-sustainable and non-biodegradable materials, such as fluoropolymers or lead containing piezoceramics, used in sensing applications. In silico genome analysis predicted genes partaking in carbohydrate metabolism, BC biogenesis, and nitrogen fixation/regulation.

Graphic abstract

中文翻译：

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一种新型细菌纤维素生产商的表征，该生产机可从包含废碳的基本介质生产环保型压电响应膜

摘要

细菌纤维素（BC）是一种可生物降解的聚合物，具有纯度，结晶度以及出色的光学，结构和机械性能。这些特性有助于BC替代例如在传感应用中使用的常规不可生物降解的材料。但是，BC的生产主要在包含模型底物和复杂的含碳化合物的常规介质中进行。为了生产环保的压电响应式BC膜，我们分离并鉴定了一种与根瘤菌相关的新型细菌菌株。该K. rhaeticusENS9a菌株在含有粗甘油的基本培养基中合成了BC，滴定度为2.9±0.3 g / L BC。据我们所知，这是未经优化的含有粗甘油的基本培养基所报告的最高BC滴度。有趣的是，由粗制甘油制得的薄膜分别显示出6-11 pC / N和40-71 pC / N的法向力和弯曲模式敏感性，证明了一个绿色平台既可解决生物工艺废物的增值问题，又可用于纤维素替代品的实现。用于传感应用的非可持续性和不可生物降解材料，例如含氟聚合物或含铅压电陶瓷。在计算机基因组分析中，预测了参与碳水化合物代谢，BC生物发生和固氮/调控的基因。

图形摘要