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Modular off-chip emulsion generator enabled by a revolving needle
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-10-28 , DOI: 10.1039/d0lc00939c
Yuxin Zhang 1, 2, 3, 4, 5 , Qianbin Zhao 6, 7, 8, 9 , Dan Yuan 6, 7, 8, 9 , Hangrui Liu 9, 10, 11, 12, 13 , Guolin Yun 6, 7, 8, 9 , Hongda Lu 6, 7, 8, 9 , Ming Li 9, 12, 13, 14 , Jinhong Guo 15, 16, 17, 18 , Weihua Li 6, 7, 8, 9 , Shi-Yang Tang 1, 2, 3, 4, 5
Affiliation  

Microfluidic chips have demonstrated unparalleled abilities in droplet generation, including precise control over droplet size and monodispersity. And yet, their rather complicated microfabrication process and operation can be a barrier for inexperienced researchers, which hinders microdroplets from unleashing their potential in broader fields of research. Here, we attempt to remove this barrier by developing an integrated and modular revolving needle emulsion generator (RNEG) to achieve high-throughput production of uniformly sized droplets in an off-chip manner. The RNEG works by driving a revolving needle to pinch the dispersed phase in a minicentrifuge tube. The system is constructed using modular components without involving any microfabrication, thereby enabling user-friendly operation. The RNEG is capable of producing microdroplets of various liquids with diameters ranging from tens to hundreds of micrometres. We further examine the principle of operation using numerical simulations and establish a simple model to predict the droplet size. Moreover, by integrating curing and centrifugation processes, the RNEG can produce hydrogel microparticles and transfer them from an oil phase into a water phase. Using this ability, we demonstrate the encapsulation and culture of single yeast cells within hydrogel microparticles. We envisage that the RNEG can become a versatile and powerful tool for high-throughput production of emulsions to facilitate diverse biological and chemical research.

中文翻译:

通过旋转针实现模块化的片外乳化液发生器

微流控芯片已显示出无与伦比的液滴生成能力,包括精确控制液滴尺寸和单分散性。然而,它们相当复杂的微加工过程和操作可能是缺乏经验的研究人员的障碍,这阻碍了微滴释放其在更广泛的研究领域中的潜力。在这里,我们尝试通过开发集成的模块化旋转式针状乳剂发生器(RNEG)来消除这种障碍,从而以片外方式实现均匀大小的液滴的高通量生产。RNEG通过驱动旋转针将分散相夹在微型离心管中而起作用。该系统使用模块化组件构建,不涉及任何微细加工,从而实现了用户友好的操作。RNEG能够产生各种液体的微滴,其直径范围从几十到数百微米。我们将使用数值模拟进一步检查操作原理,并建立一个简单的模型来预测液滴尺寸。此外,通过整合固化和离心过程,RNEG可以生产水凝胶微粒,并将其从油相转移到水相。利用这种能力,我们证明了水凝胶微粒内单个酵母细胞的封装和培养。我们设想RNEG可以成为用于高通量乳液生产的通用且功能强大的工具,以促进各种生物学和化学研究。我们将使用数值模拟进一步检查操作原理,并建立一个简单的模型来预测液滴尺寸。此外,通过整合固化和离心过程,RNEG可以生产水凝胶微粒,并将其从油相转移到水相。利用这种能力,我们证明了水凝胶微粒内单个酵母细胞的封装和培养。我们设想RNEG可以成为用于高通量乳液生产的通用且功能强大的工具,以促进各种生物学和化学研究。我们将使用数值模拟进一步检查操作原理,并建立一个简单的模型来预测液滴尺寸。此外,通过整合固化和离心过程,RNEG可以生产水凝胶微粒,并将其从油相转移到水相。利用这种能力,我们证明了水凝胶微粒内单个酵母细胞的封装和培养。我们设想RNEG可以成为用于高通量乳液生产的通用且功能强大的工具,以促进各种生物学和化学研究。我们证明了水凝胶微粒内单个酵母细胞的封装和培养。我们设想RNEG可以成为用于高通量乳液生产的通用且功能强大的工具,以促进各种生物学和化学研究。我们证明了水凝胶微粒内单个酵母细胞的封装和培养。我们设想RNEG可以成为用于高通量乳液生产的通用且功能强大的工具,以促进各种生物学和化学研究。
更新日期:2020-11-06
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