The emerging role of miRNA as regulators in esophageal squamous cell carcinoma (ESCC) progression has aroused great attention recently. In this study, the effects of miR-624-3p in ESCC progression were explored through cell proliferation, colony formation, cell cycle, and apoptosis analyses. Results showed that increased expression of miR-624-3p enhanced cancer cell viability, proliferation, migration, and invasion but inhibited apoptosis in ESCC. Moreover, luciferase reporter assay demonstrated that miR-624-3p bound to the 3′-untranslated region of phosphatase and tensin homologue (PTEN). Further study showed that miR-624-3p exerted its tumor promoting role through targeting PTEN. Taken together, these results elucidate the regulatory role of miR-624-3p in ESCC progression, shedding light on its possible clinical application in ESCC treatment.

中文翻译：

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miR-624-3p通过靶向磷酸酶和张力蛋白同源物促进食管鳞状细胞癌进展


miRNA作为食管鳞状细胞癌（ESCC）进展中的调节因子的新兴作用最近引起了人们的高度关注。在本研究中，通过细胞增殖、集落形成、细胞周期和凋亡分析探讨了 miR-624-3p 在 ESCC 进展中的影响。结果表明，miR-624-3p 表达增加可增强食管鳞癌中癌细胞的活力、增殖、迁移和侵袭，但抑制细胞凋亡。此外，荧光素酶报告基因测定表明，miR-624-3p 与磷酸酶和张力蛋白同源物 (PTEN) 的 3'-非翻译区结合。进一步研究表明miR-624-3p通过靶向PTEN发挥促癌作用。总而言之，这些结果阐明了 miR-624-3p 在 ESCC 进展中的调节作用，揭示了其在 ESCC 治疗中可能的临床应用。