Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health. GmSg-1 gene is the key enzyme gene for synthesizing class A saponins. It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level. The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1^a and GmSg-1^b genes. The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H₂O₂ (1.5 mM). It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.

中文翻译：

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基于G-四链体的杂交链反应比色检测A类大豆皂苷

大豆皂苷是种子中重要的次生代谢产物之一，对人体健康具有多种有益的生理功能。GmSg-1基因是合成A类皂苷的关键酶基因。在遗传水平上实现目视和快速检测A类皂苷具有重要意义。杂交链反应（HCR）用于视觉检测GmSg-1基因，该基因的实现是通过将目标片段的长度更改为92 bp，并使用我们设计用于检测GmSg-1 ^a和GmSg- 1个^b基因。HCR反应的最佳条件是氯高铁血红素（1.2  μ M），曲拉通X-100（0.002％），ABTS（3.8  μM）和H ₂ O ₂（1.5 mM）。研究发现，HCR对GmSg-1基因具有很高的特异性，可用于肉眼检测包含Aa型，Ab型和Aa / Ab型皂苷的不同大豆品种，为分子育种提供技术参考和理论依据。大豆的开发和功能性大豆产品的开发。