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Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction
Journal of Analytical Methods in Chemistry ( IF 2.3 ) Pub Date : 2020-11-05 , DOI: 10.1155/2020/8813239
Congcong Yin 1 , Qiaoling Zhao 1 , Aiqin Yue 2 , Weijun Du 2 , Dingbin Liu 3 , Jinzhong Zhao 1 , Yongpo Zhang 1 , Min Wang 2
Affiliation  

Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health. GmSg-1 gene is the key enzyme gene for synthesizing class A saponins. It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level. The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1a and GmSg-1b genes. The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H2O2 (1.5 mM). It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.

中文翻译:

基于G-四链体的杂交链反应比色检测A类大豆皂苷

大豆皂苷是种子中重要的次生代谢产物之一,对人体健康具有多种有益的生理功能。GmSg-1基因是合成A类皂苷的关键酶基因。在遗传水平上实现目视和快速检测A类皂苷具有重要意义。杂交链反应(HCR)用于视觉检测GmSg-1基因,该基因的实现是通过将目标片段的长度更改为92 bp,并使用我们设计用于检测GmSg-1 aGmSg- 1个b基因。HCR反应的最佳条件是氯高铁血红素(1.2  μ M),曲拉通X-100(0.002%),ABTS(3.8  μM)和H 2 O 2(1.5 mM)。研究发现,HCR对GmSg-1基因具有很高的特异性,可用于肉眼检测包含Aa型,Ab型和Aa / Ab型皂苷的不同大豆品种,为分子育种提供技术参考和理论依据。大豆的开发和功能性大豆产品的开发。
更新日期:2020-11-06
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