The objective of this study was to investigate the stability and degradation kinetics of N-acetylneuraminic acid (Neu5Ac). The pH of the solution strongly influenced the stability of Neu5Ac, which was more stable at neutral pH and low temperatures. Here, we provide detailed information on the degradation kinetics of Neu5Ac at different pH values (1.0, 2.0, 11.0 and 12.0) and temperatures (60, 70, 80 and 90 °C). The study of the degradation of Neu5Ac under strongly acidic conditions (pH 1.0–2.0) is highly pertinent for the hydrolysis of polysialic acid. The degradation kinetics of alkaline deacetylation were also studied. Neu5Ac was highly stable at pH 3.0–10.0, even at high temperature, but the addition of H2O2 greatly reduced its stability at pH 5.0, 7.0 and 9.0. Although Neu5Ac has a number of applications in products of everyday life, there are no reports of rigorous shelf-life studies. This research provides kinetic data that can be used to predict product shelf lives at different temperatures and pH values.

中文翻译：

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N-乙酰神经氨酸在不同pH值下的降解动力学和保质期

本研究的目的是研究 N-乙酰神经氨酸 (Neu5Ac) 的稳定性和降解动力学。溶液的 pH 值强烈影响 Neu5Ac 的稳定性，Neu5Ac 在中性 pH 值和低温下更稳定。在这里，我们提供了有关 Neu5Ac 在不同 pH 值（1.0、2.0、11.0 和 12.0）和温度（60、70、80 和 90 °C）下降解动力学的详细信息。Neu5Ac 在强酸性条件下（pH 1.0-2.0）降解的研究与聚唾液酸的水解高度相关。还研究了碱性脱乙酰化的降解动力学。Neu5Ac 在 pH 3.0-10.0 下非常稳定，即使在高温下也是如此，但 H2O2 的加入大大降低了其在 pH 5.0、7.0 和 9.0 下的稳定性。尽管 Neu5Ac 在日常生活产品中有许多应用，没有严格的保质期研究报告。该研究提供的动力学数据可用于预测不同温度和 pH 值下的产品保质期。