Effective adjuvants that enhance immune responses have great potential for vaccine development and disease prevention. A major challenge is the effective delivery of vaccine component to specific cellular populations in lymph nodes to boost immune response. Here, immunostimulatory DNA nanogel is designed and self-assembled with applicable size for effective lymphatic drainage and high vaccine efficacy. The efficient lymph node accumulation, enhanced dendritic cell internalization, and sustained immune stimulus of such adjuvant formulation enable potent immune activation in vitro and in vivo. Vaccination with the DNA nanogel adjuvanted OVA inhibits tumor growth and amplifies the efficacy of immunotherapy. These data indicate that programming DNA nanomaterials provides an applicable strategy for adjuvant and vaccine development. DNA nanotechnology may offer promising nanoplatforms for innovative combination immunotherapies and more therapeutic strategies.

中文翻译：

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免疫刺激性DNA纳米凝胶可实现有效的淋巴引流和高疫苗效力

增强免疫反应的有效佐剂具有开发疫苗和预防疾病的巨大潜力。一个主要的挑战是将疫苗成分有效递送到淋巴结中的特定细胞群，以增强免疫反应。在这里，免疫刺激性DNA纳米凝胶的设计和自组装具有适用的尺寸，以实现有效的淋巴引流和高疫苗效力。这种佐剂制剂的有效淋巴结积累，增强的树突状细胞内在化和持续的免疫刺激使得能够在体外和体内有效地激活免疫。用DNA纳米凝胶佐剂OVA进行疫苗接种可抑制肿瘤生长并增强免疫疗法的功效。这些数据表明，对DNA纳米材料进行编程可为佐剂和疫苗开发提供适用的策略。