Abstract

Species of the genus Aspergillus have a variety of effects on humans and have been considered industrial cell factories due to their prominent ability for manufacturing several products such as heterologous proteins, secondary metabolites, and organic acids. Scientists are trying to improve fungal strains and re-design metabolic processes through advanced genetic manipulation techniques and gene delivery systems to enhance their industrial efficiency and utility. In this review, we describe the current status of the genetic manipulation techniques and transformation methods for species of the genus Aspergillus. The host strains, selective markers, and experimental materials required for the genetic manipulation and fungal transformation are described in detail. Furthermore, the advantages and disadvantages of these techniques are described.

 抽象的

曲霉属物种对人类具有多种影响，并且由于其制造异源蛋白质、次级代谢产物和有机酸等多种产品的突出能力而被认为是工业细胞工厂。科学家们正试图通过先进的基因操作技术和基因传递系统来改进真菌菌株并重新设计代谢过程，以提高其工业效率和效用。在这篇综述中，我们描述了曲霉属物种的遗传操作技术和转化方法的现状。详细描述了遗传操作和真菌转化所需的宿主菌株、选择标记和实验材料。此外，还描述了这些技术的优点和缺点。