Abstract

Background

Analysis of allele frequencies of short tandem repeat (STR) loci in ethnically diverse populations is essential for forensic reference database construction and studies on population genetics.

Aim

To analyse genetic polymorphisms of 22 autosomal STR loci in the Serbian population and to compare them with previously published data from some European and Turkish populations.

Subjects and methods

The study was conducted among 983 unrelated individuals from Serbia. Genotyping was performed using the PowerPlex^® Fusion amplification kit. Allele frequencies and forensic parameters were calculated using FORSTAT software. Interpopulation comparisons and genetic distance calculations were performed in Arlequin and POPTREEW software.

Results

A total of 280 alleles were detected with corresponding allelic frequencies ranging from 0.0005 to 0.5255. Based on heterozygosity and the polymorphism information content, D1S1656 and Penta E may be considered as the most informative markers. Both the combined power of discrimination (CPD) and the combined power of exclusion (CPE) for the 22 analysed loci were higher than 0.999999. The combined match probability (CPM) for all 22 loci was 6.773688e⁻²⁹.

Conclusion

With respect to the results, the 22 STR loci are highly polymorphic and discriminating in the Serbian population and could be used for forensic practice and population genetics studies.

中文翻译：

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来自塞尔维亚的983个人中22个常染色体STR位点的等位基因频率和法医参数，并与其他24个人群进行比较

摘要

背景

分析不同种族的短串联重复序列（STR）基因座的等位基因频率对于法医参考数据库的建设和人群遗传学的研究至关重要。

目标

分析塞尔维亚人口中22个常染色体STR基因座的遗传多态性，并将其与某些欧洲和土耳其人口先前发表的数据进行比较。

主题与方法

这项研究是在983名来自塞尔维亚的无关人士中进行的。基因分型使用的PowerPlex执行^®融合扩增试剂盒。使用FORSTAT软件计算等位基因频率和法医参数。群体间比较和遗传距离计算在Arlequin和POPTREEW软件中进行。

结果

总共检测到280个等位基因，其对应的等位基因频率范围为0.0005至0.5255。基于杂合性和多态性信息含量，D1S1656和Penta E可能被认为是信息最丰富的标记。被分析的22个基因座的鉴别力（CPD）和排斥力（CPE）均高于0.999999。所有22个基因座的组合匹配概率（CPM）为6.773688e ^-29。

结论

关于结果，这22个STR基因座在塞尔维亚人群中具有高度多态性和区别性，可以用于法医实践和人群遗传学研究。