Abstract

AMH, KISS1R and GDF9 genes play a vital role in human and animal reproduction and might be used as the genetic markers for the reproduction traits selection. The aim of this study was to screen the single nucleotide polymorphisms (SNPs) within the AMH, KISS1R and GDF9 genes and to determine the correlations between these SNPs and the litter size in goats. Nine single SNPs within these genes were used for genotyping of the 190 Dazu black goat populations by SNaPshot technique. The polymorphisms of nine SNPs within these genes were detected in Dazu black goats. The significant correlation was observed between one SNP (g.89172108A > C) within the AMH gene and the litter size of second born in Dazu black goats (p < 0.05). The SNP was located in exon 4 (XM_018050765.1) of the AMH gene and was one nonsynonymous substitution, which resulted in a change of an amino acid from Glutamine to Proline (Gln38Pro). These results suggested that the nonsynonymous SNP g.89172108A > C of AMH gene could be used as a potential genetic marker for Marker-assisted selection (MAS) in goats breeding programs.

摘要

AMH、KISS1R和GDF9基因在人和动物的繁殖中发挥着重要作用，可作为繁殖性状选择的遗传标记。本研究的目的是筛选AMH、KISS1R和GDF9基因中的单核苷酸多态性 (SNP)，并确定这些 SNP 与山羊产仔数之间的相关性。这些基因中的9个单核苷酸多态性被用于通过SNaPshot技术对190个大足黑山羊种群进行基因分型。在大足黑山羊中检测到这些基因中9个SNP的多态性。在AMH中的一个 SNP (g.89172108A > C) 之间观察到显着相关性大足黑山羊基因与二胎产仔数( p  < 0.05)。SNP 位于AMH基因的外显子 4 (XM_018050765.1)，是一种非同义取代，导致氨基酸从谷氨酰胺变为脯氨酸 (Gln38Pro)。这些结果表明，AMH基因的非同义SNP g.89172108A > C可以作为山羊育种计划中标记辅助选择（MAS）的潜在遗传标记。