Abstract

Phytochemicals derived from plant sources are well recognized as sources of pharmacologically potent drugs in the treatment of several oxidative stress-related ailments. Dichloromethane/methanol (1:1) leaf extract of Pterocarpus mildbraedii was evaluated for its possible protection against oxidative stress and apoptosis in the liver of male Wistar rats exposed to propanil (PRP). In the experimental design, olive oil served as the vehicle, and rats were grouped into control (2 mL/kg olive oil), PRP (200 mg/kg/day), Pterocarpus mildbraedii extract (200 mg/kg/day), and Pterocarpus mildbraedii extract (200 mg/kg/day)+PRP (200 mg/kg/day), and treated daily, p.o., for seven days. Oxidative stress parameters, B-cell lymphoma 2 (Bcl-2), Bcl 2-associated X protein (Bax), p53, caspases (9/3), and terminal transferase dUTP nick end labeling (TUNEL) assays were observed in all groups. Propanil significantly elevated superoxide dismutase and lipid peroxidation levels, while concomitantly depleting GSH and p53 levels. Further, PRP enhanced the expressions of caspase-9, caspase-3, Bax, and TUNEL-positive cells in the liver of rats. However, these observed alterations were reversed following treatment with Pterocarpus mildbraedii extract. Our studies suggest that Pterocarpus mildbraedii extract protected against PRP toxicity by reducing oxidative stress and attenuating critical endpoints in the intrinsic apoptotic pathway.

摘要

源自植物的植物化学物质被公认为是治疗几种氧化应激相关疾病的药理学有效药物的来源。评估了紫檀的二氯甲烷/甲醇 (1:1) 叶提取物对暴露于丙烷 (PRP) 的雄性 Wistar 大鼠肝脏中的氧化应激和细胞凋亡的可能保护作用。在实验设计中，橄榄油作为载体，大鼠分为对照组（2 mL/kg 橄榄油）、PRP（200 mg/kg/天）、紫檀提取物（200 mg/kg/天）和紫檀提取物（200 毫克/公斤/天）+PRP（200 毫克/公斤/天），每天口服治疗 7 天。在所有组中观察到氧化应激参数、B 细胞淋巴瘤 2 (Bcl-2)、Bcl 2 相关 X 蛋白 (Bax)、p53、半胱天冬酶 (9/3) 和末端转移酶 dUTP 缺口末端标记 (TUNEL) 测定. Propanil 显着提高超氧化物歧化酶和脂质过氧化水平，同时消耗 GSH 和 p53 水平。此外，PRP 增强了大鼠肝脏中 caspase-9、caspase-3、Bax 和 TUNEL 阳性细胞的表达。然而，这些观察到的变化在用紫檀提取物处理后被逆转。我们的研究表明，紫檀提取物通过减少氧化应激和减弱内在凋亡途径中的关键终点来保护免受 PRP 毒性。